Methanogenic Community Dynamics during Anaerobic Utilization of Agricultural Wastes

This work is devoted to the investigation of the methanogenic archaea involved in anaerobic digestion of cattle manure and maize straw on the basis of terminal restriction fragment length polymorphism (T-RFLP) analysis of archaeal 16S rRNA genes. The biological diversity and dynamics of methanogenic...

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Published inActanaturae Vol. 4; no. 4; pp. 91 - 97
Main Authors Ziganshin, A M, Ziganshina, E E, Kleinsteuber, S, Pröter, J, Ilinskaya, O N
Format Journal Article
LanguageEnglish
Published Russia (Federation) A.I. Gordeyev 01.10.2012
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Summary:This work is devoted to the investigation of the methanogenic archaea involved in anaerobic digestion of cattle manure and maize straw on the basis of terminal restriction fragment length polymorphism (T-RFLP) analysis of archaeal 16S rRNA genes. The biological diversity and dynamics of methanogenic communities leading to anaerobic degradation of agricultural organic wastes with biogas production were evaluated in laboratory-scale digesters. T-RFLP analysis, along with the establishment of archaeal 16S rRNA gene clone libraries, showed that the methanogenic consortium consisted mainly of members of the generaMethanosarcinaandMethanoculleus,with a predominance ofMethanosarcinaspp. throughout the experiment.
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ISSN:2075-8251
DOI:10.32607/20758251-2012-4-4-91-97