Analysis of nutrient-dependent transcript variations in Neurospora crassa

• Published in: Fungal genetics and biology Vol. 40; no. 3; pp. 225 - 233
• Main Authors: Aign, Verena, Hoheisel, Jörg D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2003
• Subjects: Acetate metabolism; acetic acid; Correspondence analysis; Culture Media; DNA-microarray analysis; expressed sequence tags; Fungal Proteins - genetics; gene expression regulation; Gene Expression Regulation, Fungal - genetics; Genetic Variation - genetics; messenger RNA; metabolism; microarray technology; Neurospora crassa; Neurospora crassa - genetics; Neurospora crassa - growth & development; Nucleic Acid Hybridization; nutrient availability; RNA, Fungal - genetics; RNA, Fungal - isolation & purification; transcription (genetics); Transcription, Genetic - genetics; Transcriptional profiling; DNA-microarray analysis; Transcriptional profiling; Neurospora crassa; Correspondence analysis; Acetate metabolism
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/S1087-1845(03)00106-3

Nutrient-dependent variations in transcript levels of the filamentous fungus Neurospora crassa were studied on a microarray containing some 4700 cDNAs. Cells were grown in minimal and acetate medium. The isolated RNA was analyzed in comparison to the results obtained upon the hybridization of samples prepared from the RNA of cells grown in full medium. Altogether, 160 cDNA clones exhibited significant variations, falling into five distinct subgroups of very similar transcription profiles. This is indicative of the occurrence of a high degree of co-regulation of genes in N. crassa. Especially the regulation of the expression of proteins involved in metabolic pathways was found to be strongly regulated at the RNA level.