Comparative Physiological and Transcriptome Analysis Provide Insights into the Response of Cenococcum geophilum, an Ectomycorrhizal Fungus to Cadmium Stress

Cadmium (Cd) displays strong toxicity, high mobility, and cannot be degraded, which poses a serious threat to the environment. Cenococcum geophilum (C. geophilum) is one of the most common ectomycorrhizal fungi (ECMF) in the natural environment. In this study, three Cd sensitive and three Cd toleran...

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Bibliographic Details
Published inJournal of fungi (Basel) Vol. 8; no. 7; p. 724
Main Authors Shi, Yuyu, Yan, Tianyi, Yuan, Chao, Li, Chaofeng, Rensing, Christopher, Chen, Yahua, Xie, Rongzhang, Zhang, Taoxiang, Lian, Chunlan
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.07.2022
MDPI
Subjects
Antioxidants
Binding sites
Cadmium
Cell membranes
Cenococcum geophilum
Ectomycorrhizas
Enzymes
Flowers & plants
Fungi
Gene expression
Genetic diversity
Heavy metals
ion transport
Macromolecules
membrane
Metabolites
mycorrhiza
Physiology
redox
Soil contamination
Toxicity
Transcriptomes
Transcriptomics
China
Japan
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Summary:Cadmium (Cd) displays strong toxicity, high mobility, and cannot be degraded, which poses a serious threat to the environment. Cenococcum geophilum (C. geophilum) is one of the most common ectomycorrhizal fungi (ECMF) in the natural environment. In this study, three Cd sensitive and three Cd tolerant strains of C. geophilum were used to analyze the physiological and molecular responses to Cd exposure. The results showed that Cd inhibited the growth of all strains of C. geophilum but had a less toxic effect on the tolerant strains, which may be correlated to a lower content of Cd and higher activity of antioxidant enzymes in the mycelia of tolerant strains. Comparative transcriptomic analysis was used to identify differentially expressed genes (DEGs) of four selected C. geophilum strains after 2 mg/L Cd treatment. The results showed that the defense response of C. geophilum strain to Cd may be closely related to the differential expression of functional genes involved in cell membrane ion transport, macromolecular compound metabolism, and redox pathways. The results were further confirmed by RT-qPCR analysis. Collectively, this study provides useful information for elucidation of the Cd tolerance mechanism of ECMF.
Bibliography:These authors contributed equally to this work.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof8070724