PolySia-Specific Retargeting of Oncolytic Viruses Triggers Tumor-Specific Immune Responses and Facilitates Therapy of Disseminated Lung Cancer

• Published in: Cancer immunology research Vol. 3; no. 7; p. 751
• Main Authors: Kloos, Arnold, Woller, Norman, Gürlevik, Engin, Ureche, Cristina-Ileana, Niemann, Julia, Armbrecht, Nina, Martin, Nikolas T, Geffers, Robert, Manns, Michael P, Gerardy-Schahn, Rita, Kühnel, Florian
• Format: Journal Article
• Language: English
• Published: United States 01.07.2015
• Subjects: Adenoviridae - genetics; Animals; Cell Line, Tumor; Female; Genetic Therapy; Genetic Vectors - genetics; Humans; Lung Neoplasms - therapy; Mice; Mice, Inbred C57BL; Mice, Nude; Oncolytic Virotherapy; Oncolytic Viruses - genetics; Sialic Acids - genetics; T-Lymphocytes - immunology; Xenograft Model Antitumor Assays
• ISSN: 2326-6074
• DOI: 10.1158/2326-6066.CIR-14-0124-T

Polysialic acid (polySia) is expressed on several malignant tumors of neuroendocrine origin, including small cell lung cancer. In this study, we investigated the therapeutic efficacy of tumor-directed T-cell responses, elicited by polySia-retargeted oncolytic adenovirus infection, in an orthotopic murine model of disseminated polySia-positive lung cancer. In several cell lines, we demonstrated highly polySia-selective retargeting of adenoviral infection using a bispecific adapter comprising the ectodomain of the coxsackievirus/adenovirus receptor and a polySia-recognizing single-chain antibody domain. PolySia-dependent systemic infection in vivo facilitated effective uptake of viruses in subcutaneous polySia-expressing human tumors, whereas hepatic viral load and hepatotoxicity were significantly reduced. The impact and nature of antitumoral immune responses triggered by systemic delivery of polySia-retargeted oncolytic adenoviruses were investigated in an orthotopic model of disseminated lung cancer. Interestingly, improved transduction by polySia-retargeted oncolytic adenoviruses led to CD45-positive cell infiltrates in close association with large lytic areas. Consistently, enhanced tumor regression and prolonged survival was only observed in immunocompetent mice, but not in T-cell-deficient mice. To investigate whether improved systemic infection by polySia retargeting would elicit a tumor-specific T-cell response, we screened the used lung cancer cells for mutated oncogenes by complete exon sequencing. In agreement with our other results, only retargeted oncolysis was able to induce a significant response specific for the tumor-associated neoepitope Gsta2-Y9H. In conclusion, we demonstrated that effective retargeting of oncolytic adenovirus against polySia-expressing tumors elicits an effective tumor-directed T-cell response after systemic virus delivery and facilitates therapy of disseminated lung cancer.