Electropolymerized biotinylated poly (pyrrole–viologen) film as platform for the development of reagentless impedimetric immunosensors

• Published in: Electrochemistry communications Vol. 12; no. 2; pp. 311 - 314
• Main Authors: Gondran, Chantal, Orio, Maylis, Rigal, David, Galland, Bruno, Bouffier, Laurent, Gulon, Tioga, Cosnier, Serge
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.02.2010; Amsterdam Elsevier; New York, NY
• Subjects: Analytical chemistry; Biological and medical sciences; Biosensors; Biotechnology; Biotin; Chemical Sciences; Fundamental and applied biological sciences. Psychology; Immunosensor; Impedance; Methods. Procedures. Technologies; Polypyrrole; Various methods and equipments; Viologen; Viologen; Biotin; Impedance; Polypyrrole; Immunosensor; Chemical analysis; Nyquist diagram; Vibrio cholerae; Pyrrole derivative polymer; Electrochemical polymerization; Viologen polymer; Electrodes; Bacteria; Electrochemical reaction; Biotinylation; Antibody; Impedancemetry; Carbon; Electrochemical detector; Conducting polymers; Polyelectrolyte; Toxin; Biosensor; Preparation; Vibrionaceae; Glassy state; Electrochemical impedance spectroscopy
• ISSN: 1388-2481; 1873-1902
• DOI: 10.1016/j.elecom.2009.12.026

We describe herein the synthesis and electrochemical polymerization of a viologen monomer functionalized by a biotin group and the use of its redox properties for the impedimetric sensing of protein binding. The electrochemical oxidation of the pyrrole group at 0.85 V in CH 3CN led to a biotinylated polypyrrole film allowing the successive anchoring of an avidin layer and a biotinylated cholera toxin layer. EIS performed at −0.45 V/SCE without redox probe in solution showed the specific immobilization of the anti-cholera toxin antibody.