Biochemical responses in seabream (Sparus aurata) caged in-field or exposed to benzo(a)pyrene and paraquat. Characterization of glutathione S-transferases

• Published in: Ecotoxicology and environmental safety Vol. 88; pp. 169 - 177
• Main Authors: Jebali, Jamel, Chicano-Gálvez, Eduardo, Banni, Mohamed, Guerbej, Hamadi, Boussetta, Hamadi, López-Barea, Juan, Alhama, José
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.02.2013; Elsevier
• Subjects: Affinity chromatography; Animal and plant ecology; Animal, plant and microbial ecology; Animals; Antioxidant enzymes; antioxidants; Applied ecology; Benzo(a)pyrene - toxicity; Biological and medical sciences; biomarkers; Biomarkers - metabolism; Biotransformation; Biotransformation enzymes; catalase; Cytochrome P-450 CYP1A1 - metabolism; ecosystems; Ecotoxicology, biological effects of pollution; Enzymes - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic - drug effects; General aspects; glucose 6-phosphate; glutathione; Glutathione - metabolism; glutathione peroxidase; glutathione transferase; Glutathione Transferase - metabolism; glutathione-disulfide reductase; Isoforms; Oxidative Stress - drug effects; paraquat; Paraquat - toxicity; proteins; Proteomics; Sea Bream - physiology; Sea water ecosystems; Sparus aurata; Synecology; Time Factors; two-dimensional gel electrophoresis; Water Pollutants, Chemical - toxicity; Affinity chromatography; Antioxidant enzymes; Biotransformation enzymes; Isoforms; Toxicity; Systemic; Characterization; Marine environment; Persistent organic pollutant; Sparus aurata; Glutathione transferase; Pisces; Biotransformation; Organic compounds; Hydrocarbon; Benzopyrene; Ecotoxicology; Enzyme; Transferases; Pesticides; Detoxification; Antioxidant; Polycyclic aromatic compound; Herbicide; Quaternary ammonium compound; Vertebrata; Aminoacid; Affinity; Environment; Paraquat
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2012.11.013

Gilthead seabream (Sparus aurata) specimens were caged in-field at the Téboulba harbour or exposed to benzo(a)pyrene [B(a)P] or to paraquat [PQ] plus B(a)P, and several biochemical biomarker responses were investigated. Antioxidant enzymes, such as glutathione peroxidase, catalase and glutathione reductase, significantly increased in the in-field and B(a)P+PQ exposures, but were only moderately affected by B(a)P alone. Glucose-6-phosphate and 6-phosphogluconate dehydrogenases significantly diminished after in-field exposure. Different responses with biotransformation enzymes were observed: the P4501A-associated EROD activity was highly induced in response to B(a)P and B(a)P+PQ exposures, while total activity of the glutathione S-transferase (GST) was similar to control. However, after purification of the GST proteins by affinity chromatograpy and analysis by two-dimensional electrophoresis, nineteen highly reproducible isoforms were resolved. In addition, some of reproducible isoforms showed different and specific expression patterns in response to contaminants. Thus, proteomic analysis of the purified GST subunits is a reliable tool for ecotoxicological research, useful in polluted marine ecosystem as an effective biomarker of contamination. ► Enzymatic and none enzymatic biomarkers were investigated in Sparus aurata. ► Total GST activity was less sensitive to contaminant exposures. ► GST subunits show different expression in response to contaminants.