Expression of an Entire Bacterial Operon in Plants

• Published in: Plant physiology (Bethesda) Vol. 158; no. 4; pp. 1883 - 1892
• Main Authors: Mozes-Koch, Rita, Gover, Ofer, Tanne, Edna, Peretz, Yuval, Maori, Eyal, Chernin, Leonid, Sela, Ilan
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.04.2012
• Subjects: Bacteria; Biochemical pathways; Biological and medical sciences; Chromatography, High Pressure Liquid; Disease Resistance - immunology; DNA Replication - genetics; Fundamental and applied biological sciences. Psychology; Geminivirus; Gene Expression Regulation, Bacterial; Genes; Genes, Bacterial - genetics; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Lycopersicon esculentum - immunology; Lycopersicon esculentum - microbiology; Mass Spectrometry; Messenger RNA; Metabolic diseases; Metabolites; Operon - genetics; Operons; Plant Diseases - immunology; Plant Diseases - microbiology; Plant physiology and development; Plant roots; Plants; Plastids; Pseudomonas fluorescens - genetics; Pyrrolnitrin - chemistry; Pyrrolnitrin - metabolism; Rhizoctonia - physiology; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Solanum; SYSTEMS BIOLOGY, MOLECULAR BIOLOGY, AND GENE REGULATION; Tomato yellow leaf curl virus; Plant physiology; Operon; Bacteria
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.111.186197

Multigene expression is required for metabolic engineering, i.e. coregulated expression of all genes in a metabolic pathway for the production of a desired secondary metabolite. To that end, several transgenic approaches have been attempted with limited success. Better success has been achieved by transforming plastids with operons. IL-60 is a platform of constructs driven from the geminivirus Tomato yellow leaf curl virus. We demonstrate that IL-60 enables nontransgenic expression of an entire bacterial operon in tomato (Solarium lycopersicum) plants without the need for plastid (or any other) transformation. Delivery to the plant is simple, and the rate of expressing plants is close to 100%, eliminating the need for selectable markers. Using this platform, we show the expression of an entire metabolic pathway in plants and delivery of the end product secondary metabolite (pyrrolnitrin). Expression of this unique secondary metabolite resulted in the appearance of a unique plant phenotype disease resistance. Pyrrolnitrin production was already evident 2 d after application of the operon to plants and persisted throughout the plant's life span. Expression of entire metabolic pathways in plants is potentially beneficial for plant improvement, disease resistance, and biotechnological advances, such as commercial production of desired metabolites.