Development of a lateral flow immunoassay of C-reactive protein detection based on red fluorescent nanoparticles

• Published in: Analytical biochemistry Vol. 556; pp. 129 - 135
• Main Authors: Cai, Yanxue, Kang, Keren, Liu, Yujia, Wang, Yu, He, Xiaowei
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2018
• Subjects: Antibodies, Monoclonal - chemistry; C-reactive protein; C-Reactive Protein - analysis; Chromatography, Affinity - methods; Dye-labeled nanoparticles; Humans; Immunoassay; Immunoassay - methods; Limit of Detection; Nanoparticles - chemistry; Oxazines - chemistry; Point-of-care tests; Reproducibility of Results; Immunoassay; Point-of-care tests; Dye-labeled nanoparticles; C-reactive protein
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2018.06.017

We have developed a reliable and rapid immunoassay based on a facile synthesis of fluorescent nanoparticles integrated in immunochromatography technique to quantitatively detect C-reactive protein (CRP). The method is based on a sandwich immunoassay using the Nile-red doped nanoparticles/CRP monoclonal antibody conjugate. The method is simple and fast, with a detection limit of 0.091 mg/L. It provides quantitative analysis in the range of 0.1–160 mg/L, which is adequate for detecting CRP of acute inflammatory or cardiovascular disease. This strategy displayed a good reproducibility and stability to straightforwardly analyze the plasma samples without complicated washing steps, thereby reducing the operating procedures for non-professionals and promoting the detection efficiency and the whole detection process can be completed in 3 min. This approach for carrying out immunoassays can be applied to the detection of CRP in the point-of-care tests. [Display omitted] Schematic presentation of lateral flow immunoassays (LFIA) based on the use of red fluorescent nanoparticles (RPNs) as signaling labels for the rapid determination of C-reactive protein (CRP) in plasma samples and the core-shell structure of the RPNs. •A facile one-step synthesis is used to prepare single microspheres for CRP assay.•Clear core-shell structures of microspheres contribute to divide functional areas.•Separating antibody and dyes to improve the sensitivity and stability of test strip.•An analysis of clinical samples is used to get the reliable assay results.