Production of Macrophage Migration Inhibitory Factor by Human and Murine Neuroblastoma

Tumor cells avoid immune recognition by subverting the ability of the immune system to mount an inflammatory response that generates cytotoxic effector cells. This can be achieved through cytokine production by the tumor itself. Our objective was to determine the cytokine profile of neuroblastoma (N...

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Published inTumor biology Vol. 23; no. 3; pp. 123 - 129
Main Authors Bin, Qian, Johnson, Bryon D., Schauer, Dennis W., Casper, James T., Orentas, Rimas J.
Format Journal Article
LanguageEnglish
Published Basel, Switzerland Karger 01.05.2002
Springer Nature B.V
Subjects
Animals
Biological and medical sciences
Cell Migration Inhibition
Humans
Interleukin-6 - analysis
Macrophage Migration-Inhibitory Factors - biosynthesis
Medical sciences
Mice
Neuroblastoma - metabolism
Neurology
Research Article
RNA, Messenger - analysis
Tumor Cells, Cultured
Tumors of the nervous system. Phacomatoses
Macrophage migration inhibitory factor
Tumor-derived cytokines
MIF
Neuroblastoma
Human
Nervous system diseases
Immune response
Immunomodulation
Secretion
Cytokine
Rodentia
Malignant tumor
Carcinogenesis
Interleukin 6
Cell motility
Vertebrata
Mammalia
Mouse
Animal
Migration inhibitory factor
Established cell line
Tumor progression
Autonomic neuropathy
Macrophage
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Summary:Tumor cells avoid immune recognition by subverting the ability of the immune system to mount an inflammatory response that generates cytotoxic effector cells. This can be achieved through cytokine production by the tumor itself. Our objective was to determine the cytokine profile of neuroblastoma (NB) lesions in tumor vaccine models. We found that the murine NB cell line, Neuro2a, secretes macrophage migration inhibitory factor, MIF, a multifunctional cytokine with the potential to block effective immune responses to a tumor. Patient-derived NB cell lines were also found to produce MIF. MIF production by NB was documented at the level of RNA by RNAse protection, soluble cytokine production by ELISA, and in a macrophage migration assay. Our studies also confirmed reports of IL-6 production by human NB cell lines. NB culture-derived MIF was also shown to activate tumor cell migration. This supports the hypothesis that MIF is a tumor-derived cytokine that may play a role in NB aggressiveness and evasion of immune recognition.
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ISSN:1010-4283
1423-0380
DOI:10.1159/000064028