Wrinkled petals and stamens 1, is required for the morphogenesis of petals and stamens in Lotus japonicus

• Published in: Cell research Vol. 16; no. 5; pp. 499 - 506
• Main Authors: Chen, Jiang Hua, Pang, Ji Liang, Wang, Li Lin, Luo, Yong Hai, Li, Xin, Cao, Xiang Ling, Lin, Kui, Ma, Wei, Hu, Xiao He, Luo, Da
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.05.2006
• Subjects: Developmental stages; Flowers; Flowers - genetics; Flowers - growth & development; Flowers - ultrastructure; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant - genetics; In Situ Hybridization; Loteae - genetics; Loteae - growth & development; Loteae - ultrastructure; Lotus japonicus; Microscopy, Electron, Scanning; Morphogenesis - genetics; Mutants; Mutation - genetics; Organs; Phenotype; Plant Proteins - genetics; Plant Proteins - metabolism
• ISSN: 1001-0602; 1748-7838
• DOI: 10.1038/sj.cr.7310061

Although much progress has been made in understanding how floral organ identity is determined during the floral development, less is known about how floral organ is elaborated in the late floral developmental stages. Here we describe a novel floral mutant, wrinkled petals and stamens1 （wps1）, which shows defects in the development of petals and stamens. Genetic analysis indicates that wpsl mutant is corresponding to a single recessive locus at the long arm of chromosome 3. The early development of floral organs in wpsl mutant is similar to that in wild type, and the malfunction of the mutant commences in late developmental stages, displaying a defect on the appearance of petals and stamens. In the mature flower, petals and stamen filaments in the mutant are wrinkled or folded, and the cellular morphology under L1 layer of petals and stamen filaments is abnormal. It is found that the expression patterns of floral organ identity genes are not affected in wpsl mutants compared with that of wild type, consistent with the unaltered development of all floral organs. Furthermore, the identities of epidermal cells in different type of petals are maintained. The histological analysis shows that in wpsl flowers all petals are irregularly folded, and there are knotted structures in the petals, while the shape and arrangement of inner cells are malformed and unorganized. Based on these results, we propose that Wpsl acts downstream to the class B floral organ identity genes, and functions to modulate the cellular differentiation during the late flower developmental stages.