Detection of LINE RT elements in the olive flounder (Paralichthys olivaceus) genome and expression analysis after infection with S. parauberis

• Published in: Genes & genomics Vol. 38; no. 11; pp. 1105 - 1110
• Main Authors: Gim, Jeong-An, Nam, Gyu-Hwi, Kim, Woo-Jin, Lee, Hee-Eun, Mishra, Anshuman, Choe, Yunjeong, Park, Chan-Il, Kim, Do-Hyung, Choi, Yung Hyun, Cha, Hee-Jae, Yoon, Dahye, Kim, Suhkmann, Kim, Heui-Soo
• Format: Journal Article
• Language: English
• Published: Seoul The Genetics Society of Korea 01.11.2016; Springer Nature B.V; 한국유전학회
• Subjects: Aldehydes; Amino acid sequence; amino acid sequences; Animal Genetics and Genomics; Biomedical and Life Sciences; Dicentrarchus labrax; Fish; flounder; Gasterosteus aculeatus; genome; Genomes; grouper; Human Genetics; Life Sciences; Long interspersed nucleotide elements; Microbial Genetics and Genomics; Nucleotide sequence; Paralichthys olivaceus; pathogens; Plant Genetics and Genomics; Pleuronectiformes; Polymerase chain reaction; Research Article; retrotransposons; reverse transcriptase polymerase chain reaction; Reverse transcription; RNA-directed DNA polymerase; sequence analysis; Spleen; Streptococcus parauberis; 생물학; Expression; Olive flounder; Long interspersed nuclear elements
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-016-0457-8

Long interspersed nuclear elements (LINEs) are widely distributed in the vertebrate genome, and can be either beneficial or detrimental to the host genome. Here we identified three members of LINE RT elements in the olive flounder genome. They showed high amino acid sequence identity (89–99 %), and the sequences of LINE reverse transcriptase (RT) in olive flounder are closely related to those of coral grouper, European seabass, and three-spined stickleback. Real-time reverse transcription-polymerase chain reaction analysis indicated that expression of the OF (Olive flounder)-LINE Chr3-1 RT increased more in spleen than in other tissues after treatment with the pathogen Streptococcus parauberis . These data may form the basis for further studies on the function of retroelements in infected olive flounder.