Fluorescence-detected three-dimensional linear dichroism: A method to determine absorption anisotropy in single sub-wavelength size nanoparticles

Two illumination modes in fluorescence microscope provide full absorption anisotropy of luminescent nanoparticles. We introduce theoretically and demonstrate experimentally a method for the determination of three-dimensional absorption anisotropy (three-dimensional linear dichroism) of single lumine...

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Bibliographic Details
Published inChemical physics letters Vol. 487; no. 4; pp. 312 - 314
Main Authors Furumaki, Shu, Habuchi, Satoshi, Vacha, Martin
Format Journal Article
LanguageEnglish
Published Elsevier B.V 05.03.2010
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Summary:Two illumination modes in fluorescence microscope provide full absorption anisotropy of luminescent nanoparticles. We introduce theoretically and demonstrate experimentally a method for the determination of three-dimensional absorption anisotropy (three-dimensional linear dichroism) of single luminescent nanoparticles. The anisotropy is measured by monitoring polarization-modulated fluorescence intensity excited alternately by epi-illumination and by total internal reflection. As an example of the application of the method, three-dimensional linear dichroism measured on single light-harvesting systems of photosynthetic bacteria is presented.
ISSN:0009-2614
1873-4448
DOI:10.1016/j.cplett.2010.01.055