Effects of Chitooligosaccharide Lactate Salt on Activity of Acetaldehyde Dehydrogenase

• Published in: Journal of medicinal food Vol. 13; no. 5; pp. 1061 - 1068
• Main Authors: Cho, Si Young, Yun, Jun-Won, Park, Pil Joon, Sohn, Jong Hee, Seo, Dae-Bang, Lim, Kyung Min, Kim, Wan Gi, Lee, Sang-Jun
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.10.2010
• Subjects: acetaldehyde; Acetaldehyde - blood; acetaldehyde dehydrogenase; Alcohol Dehydrogenase - metabolism; alcohols; Aldehyde Dehydrogenase - genetics; Aldehyde Dehydrogenase, Mitochondrial; Aldehyde Oxidoreductases - antagonists & inhibitors; Aldehyde Oxidoreductases - metabolism; Animals; blood; Cell Survival - drug effects; chitin; chitooligosaccharides; chitosan; Chitosan - pharmacology; cytotoxicity; daidzin; Enzyme Inhibitors - pharmacology; Ethanol - blood; Ethanol - toxicity; Hep G2 Cells; human cell lines; Humans; Isoflavones - pharmacology; Lactates - pharmacology; Liver - enzymology; Male; Mice; Mice, Hairless; Mitochondria, Liver - enzymology; Oligosaccharides - pharmacology; oral administration; reactive oxygen species; Reactive Oxygen Species - metabolism; RNA, Messenger - analysis
• ISSN: 1096-620X; 1557-7600
• DOI: 10.1089/jmf.2009.1323

Chitooligosaccharides (COS), a kind of oligosaccharide made from chitin or chitosan, have been used a popular remedy for hangovers. In this study we investigated the in vitro effect of COS lactate salt on ethanol-induced cytotoxicity and the in vivo effect of short-term COS lactate salt feeding on ethanol-induced hangover. Pretreatment of HepG2 cells with COS lactate salt significantly reduced ethanol-induced cytotoxicity and suppressed generation of reactive oxygen species. In addition, COS lactate salt dose-dependently increased acetaldehyde dehydrogenase (ALDH) activity in vitro and reversed the ALDH inhibition induced by daidzin. Furthermore, oral administration of COS lactate salt (200 mg/kg) for 5 days significantly decreased the blood levels of alcohol and acetaldehyde in ethanol-treated mice. It was also demonstrated that hepatic mitochondrial ALDH activity was significantly increased in COS lactate salt-treated mice. Taken together, these findings indicate that COS lactate salt may have efficacy for the management of alcoholic hangovers.