Identification of a novel candidate gene for rolled leaf in rice

• Published in: Genes & genomics Vol. 38; no. 11; pp. 1077 - 1084
• Main Authors: Lee, Yoon Kyung, Woo, Mi-Ok, Lee, Dongryung, Lee, Gileung, Kim, Backki, Koh, Hee-Jong
• Format: Journal Article
• Language: English
• Published: Seoul The Genetics Society of Korea 01.11.2016; Springer Nature B.V; 한국유전학회
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Chromosome 1; chromosomes; cultivars; Ethyl methanesulfonate; Gene mapping; Genetic analysis; Genotype & phenotype; Human Genetics; introns; Leaves; Life Sciences; Microbial Genetics and Genomics; Morphogenesis; Mutagenesis; mutants; Oryza sativa; phenotype; Plant Genetics and Genomics; Polymerase chain reaction; recessive genes; Research Article; reverse transcriptase polymerase chain reaction; rice; sequence analysis; Serine-tRNA ligase; Single-nucleotide polymorphism; Splicing; 생물학; Leaf rolling; Mapping; Bulliform cell; NGS; Rice
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-016-0451-1

A semi-narrow and adaxially rolled leaf mutant, rl15(t) , was induced from Korean japonica rice cultivar Ilpum by chemical mutagenesis using ethyl methanesulfonate. We characterized the mutant and identified the novel gene causing the mutant phenotype. Cytological analysis of mutant leaves indicated that the adaxial leaf-rolling phenotype is due to the reduced size and number of bulliform cells in the mutant. Genetic analysis showed that the rolled leaf trait is controlled by a single recessive gene, designated rl15(t) . Using an F 2 mapping population generated from a cross between Milyang23 and the mutant, we mapped the candidate region to a 174 kb interval on the long arm of chromosome 1 near the centromeric region. Through whole genome sequencing in bulk and MutMap analysis, we identified the causal SNP within the candidate region. The results of RT-PCR analysis indicated that a splicing error occurred due to a base change from G to A at the beginning of the fifth intron of LOC_Os01g37837 , which encodes a putative seryl-tRNA synthetase, resulting in the mutant phenotype. Further study of the rl15(t) gene will facilitate analysis of leaf architecture and morphogenesis in rice plants.