Structural analysis, molecular docking and molecular dynamics of an edematogenic lectin from Centrolobium microchaete seeds

• Published in: International journal of biological macromolecules Vol. 117; pp. 124 - 133
• Main Authors: Neco, Antonio Hadson Bastos, Pinto-Junior, Vanir Reis, Araripe, David Alencar, Santiago, Mayara Queiroz, Osterne, Vinicius Jose Silva, Lossio, Claudia Figueiredo, Nobre, Clareane Avelino Simplicio, Oliveira, Messias Vital, Silva, Mayara Torquato Lima, Martins, Maria Gleiciane Queiroz, Cajazeiras, Joao Batista, Marques, Gabriela Fernandes Oliveira, Costa, Diego Rabelo, Nascimento, Kyria Santiago, Assreuy, Ana Maria Sampaio, Cavada, Benildo Sousa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2018
• Subjects: CML; Dalbergieae; Inflammatory; Molecular dynamics; Structure; Dalbergieae; Molecular dynamics; Inflammatory; CML; Structure
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.05.166

Lectins represent a class of proteins or glycoproteins capable of reversibly binding to carbohydrates. Seed lectins from the Dalbergieae tribe (Leguminosae) have structural variability, carbohydrate specificity, and biological effects, such as inflammation, vasorelaxation and cancer antigen binding. To comprehensively address these factors, the present work aimed to establish and characterize the three-dimensional structure of Centrolobium microchaete lectin (CML) by homology modeling, investigate protein-carbohydrate interactions and evaluate its inflammatory effect on mice. Molecular docking was performed to analyze interactions of the lectin with monosaccharides, disaccharides and N-glycans. Two dimannosides, methyl mannose-1,3-α-D-mannose (MDM) and mannose-1,3-α-D-mannose (M13), were used in molecular dynamics (MD) simulations to study the behavior of the carbohydrate-recognition domain (CRD) over time. Results showed an expanded domain within which hydrophobic interactions with the methyl group in the MDM molecule were established, thus revealing novel interactions for mannose-specific Dalbergieae lectins. To examine its biological activities, CML was purified in a single step by affinity chromatography on Sepharose-mannose matrix. The lectin demonstrated inflammatory response in the paw edema model and stimulated leukocyte migration to the animal peritoneal cavities, an effect elicited by CRD. For the first time, this work reports the molecular dynamics of a lectin from the Dalbergieae tribe. •We obtained CML three-dimensional structure by homology modeling.•Molecular docking and dynamics analysis of CML structure was performed.•CRD volume and cavity of CML was analyzed.•We evaluated the inflammatory effect of CML in mice.