Presumptive identification of Mycobacterium tuberculosis complex based on cord formation in BACTEC MGIT 960 medium

We considered samples received for culture of mycobacteria using BACTEC MGIT 960 system over a period of 1 year. Tubes flagged positive by MGIT were evaluated for presence of serpentine cording. The cord formation was compared with isolates identified as Mycobacterium tuberculosis complex (MTC) base...

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Published inIndian journal of medical microbiology Vol. 30; no. 2; pp. 218 - 221
Main Authors Singhal, R, Arora, J, Bhalla, M, Lal, P, Reza, S, Behera, D, Myneedu, VP
Format Journal Article
LanguageEnglish
Published India Elsevier B.V 01.04.2012
Elsevier Limited
Subjects
Bacteriological Techniques - methods
Cord
Culture Media - chemistry
Gram-positive bacteria
Humans
Identification systems
Immunization
Laboratories
Microbiology
Microscopy - methods
mycobacterium tuberculosis
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - cytology
Mycobacterium tuberculosis - growth & development
Mycobacterium tuberculosis - isolation & purification
p-nitrobenzoic acid
Quality control
Sensitivity and Specificity
Tuberculosis
India
p-nitrobenzoic acid
Cord
mycobacterium tuberculosis
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Summary:We considered samples received for culture of mycobacteria using BACTEC MGIT 960 system over a period of 1 year. Tubes flagged positive by MGIT were evaluated for presence of serpentine cording. The cord formation was compared with isolates identified as Mycobacterium tuberculosis complex (MTC) based on p-nitrobenzoic acid (PNB) test. Cords were found in 591 isolates of which 584 (98.8%) were confirmed as MTC. The sensitivity and specificity of cord formation were found to be 99.7% and 89.9%, respectively.
ISSN:0255-0857
1998-3646
DOI:10.4103/0255-0857.96697