Systemic and local reactions of a water-soluble copolymer bone on a bony defect of rabbit model

• Published in: Surgical neurology Vol. 72; pp. S75 - S79
• Main Authors: Lee, Tao-Chen, MD, Chang, Nyuk-Kong, DVM, Su, Feng-Wen, MD, Yang, Yu-Lin, PhD, Su, Thung-Ming, MD, Lin, Yu-Jun, MD, Lin, Wan-Ching, BS, Huang, Hsiu-Yu, MD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2009
• Subjects: Animals; Biomarkers - analysis; Biomarkers - metabolism; Bone Diseases - surgery; Bone hemostatic agent; Bone Substitutes - chemistry; Bone Substitutes - toxicity; Bone wax; C-Reactive Protein - analysis; C-Reactive Protein - metabolism; Disease Models, Animal; Drug Combinations; Inflammation - chemically induced; Inflammation - physiopathology; Kidney Diseases - chemically induced; Kidney Diseases - physiopathology; Leukocyte Count; Male; Neurology; Ostene; Palmitates - therapeutic use; Poloxamer - chemistry; Poloxamer - toxicity; Polymers - chemistry; Polymers - toxicity; Rabbits; Reconstructive Surgical Procedures - methods; Surgery; Waxes - therapeutic use; CRP; erythrocyte sedimentation rate; C-reactive protein; white blood cell; ESR; Ostene; Bone hemostatic agent; Bone wax; WBC; BUN; blood urea nitrogen
• ISSN: 0090-3019; 1879-3339
• DOI: 10.1016/j.surneu.2009.06.007

Abstract Background Ostene®, a synthetic water-soluble bone hemostatic agent, is commercially available. In the current study, we evaluated the systemic and local effects of this copolymer in a rabbit model. Methods Eighteen rabbits underwent creation of a bony defect at right iliac crest. These rabbits were then evenly divided into 3 groups. In group 1, the defect surfaces were treated with bone wax; in group 2, the defect surfaces were treated with Ostene®; in group 3, the defect surfaces were not treated with anything. Then, the animals underwent blood examinations, including WBC count, CRP, and ESR at 0, 1, 3, and 6 weeks, and were killed at 6 weeks for histologic examination. Another 6 rabbits (group 4) underwent the same surgical treatment of group 2 animals but had blood examinations of BUN and creatinine. Results The blood examinations showed that the WBC count, CRP, and ESR of all the animals in the first 3 groups were within normal limits in the postoperative periods. Microscopic examinations demonstrated residual bone wax and fibrotic tissue at the defect surfaces in group 1 animals. However, there was no Ostene® at the defect surfaces in group 2 animals. The groups 2 and 3 animals showed no fibrotic tissue at the defect surfaces. The group 4 animals showed normal serum levels of BUN and creatinine in the postoperative periods. Conclusion Ostene® is absorbable and induces no systemic inflammation (including acute renal damage) and local inflammation in animal bodies.