Assembly of functionally active Drosophila origin recognition complex from recombinant proteins

In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped...

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Bibliographic Details
Published inGenes & development Vol. 13; no. 10; pp. 1289 - 1296
Main Authors Chesnokov, I, Gossen, M, Remus, D, Botchan, M
Format Journal Article
LanguageEnglish
Published United States Cold Spring Harbor Laboratory Press 15.05.1999
Subjects
Amino Acid Sequence
Animals
Chromosome Mapping
Cloning, Molecular
DNA Replication - physiology
DNA-Binding Proteins - analysis
DNA-Binding Proteins - genetics
Drosophila
Drosophila - genetics
Male
Molecular Sequence Data
Origin Recognition Complex
Ovum - metabolism
Recombinant Fusion Proteins - genetics
Research Paper
Sequence Homology, Amino Acid
Spermatozoa - metabolism
Time Factors
Xenopus
Xenopus - metabolism
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Summary:In eukaryotes the sites for the initiation of chromosomal DNA replication are believed to be determined in part by the binding of a heteromeric origin recognition complex (ORC) to DNA. We have cloned the genes encoding the subunits of the Drosophila ORC. Each of the genes is unique and can be mapped to discrete chromosomal locations implying that the pattern and developmental regulation of origin usage in Drosophila is not regulated solely by a large family of different ORC proteins. The six-subunit ORC can be reconstituted with recombinant proteins into a complex that restores DNA replication in ORC-depleted Drosophila or Xenopus egg extracts.
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Corresponding author.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.13.10.1289