Occurrence of antimicrobial resistance and antimicrobial resistance genes in methicillin-resistant Staphylococcus aureus isolated from healthy rabbits

• Published in: Veterinary World Vol. 15; no. 11; pp. 2699 - 2704
• Main Authors: Jangsangthong, Arunee, Suriyakhun, Nawarat, Tunyong, Witawat, Kong-Ngoen, Thida, Santajit, Sirijan, Indrawattana, Nitaya, Buranasinsup, Shutipen
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.11.2022
• Subjects: antimicrobial resistance; antimicrobial-resistant genes; healthy rabbits; methicillin-resistant staphylococcus aureus; healthy rabbits; methicillin-resistant Staphylococcus aureus; antimicrobial resistance; antimicrobial-resistant genes
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2022.2699-2704

Methicillin-resistant globally, (MRSA) is a major cause of disease in both humans and animals. Several studies have documented the presence of MRSA in healthy and infected animals. However, there is less information on MRSA occurrence in exotic pets, especially healthy rabbits. This study aimed to look into the antimicrobial resistance profile, hidden antimicrobial-resistant genes in isolated bacteria, and to estimate prevalence of MRSA in healthy rabbits. Two-hundreds and eighteen samples, including 42 eyes, 44 ears, 44 oral, 44 ventral thoracic, and 44 perineal swabs, were taken from 44 healthy rabbits that visited the Prasu-Arthorn Animal Hospital, in Nakornpathom, Thailand, from January 2015 to March 2016. The traditional methods of Gram stain, mannitol fermentation, hemolysis on blood agar, catalase test, and coagulase production were used to confirm the presence of in all specimens. All bacterial isolates were determined by antimicrobial susceptibility test by the disk diffusion method. The polymerase chain reaction was used to identify the antimicrobial-resistant genes ( Z, A, A D, A, K, A, A, and G) in isolates of MRSA with a cefoxitin-resistant phenotype. From 218 specimens, 185 were isolated, with the majority of these being found in the oral cavity (29.73%) and ventral thoracic area (22.7%), respectively. Forty-seven (25.41%) MRSAs were found in isolates, with the majority of these being found in the perineum (16, 34.04%) and ventral thoracic area (13, 27.66%) specimens. Among MRSAs, 29 (61.7%) isolates were multidrug-resistant (MDR) strains. Most of MRSA isolates were resistant to penicillin (100%), followed by ceftriaxone (44.68%) and azithromycin (44.68%). In addition, these bacteria contained the most drug-resistance genes, Z (47.83%), followed by A (36.17%) and K (23.4%). This study revealed that MRSA could be found even in healthy rabbits. Some MRSAs strains were MDR-MRSA, which means that when an infection occurs, the available antibiotics were not effective in treating it. To prevent the spread of MDR-MRSA from pets to owners, it may be helpful to educate owners about effective prevention and hygiene measures.