Fluorescence quenching of fulvic acids by fullerene in water

• Published in: Environmental pollution (1987) Vol. 172; pp. 100 - 107
• Main Authors: Wu, Fengchang, Bai, Yingchen, Mu, Yunsong, Pan, Bo, Xing, Baoshan, Lin, Ying
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.01.2013; Elsevier
• Subjects: Applied sciences; aquatic ecosystems; Association constant; Benzopyrans - chemistry; biogeochemical cycles; Biological and physicochemical phenomena; Earth sciences; Earth, ocean, space; Ecosystem; Engineering and environment geology. Geothermics; Exact sciences and technology; Fluorescence; fluorescence emission spectroscopy; Fresh Water - chemistry; Fullerene; Fullerenes - chemistry; fulvic acids; Humic substance; hydrophobicity; Models, Chemical; Natural water pollution; Pollution; Pollution, environment geology; Spectrometry, Fluorescence; titration; Water Pollutants, Chemical - chemistry; Water treatment and pollution; Fullerene; Fluorescence; Association constant; Humic substance; Pollutant behavior; Atomic cluster; Organic acids; Fluorescence spectrometry; Intermolecular interaction; Fullerenes; Water pollution; Fulvic acid; Nanostructured materials; Ultraviolet visible spectrometry
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2012.08.005

Fullerene can be suspended in water as nanoscaled-fullerene-aggregates (nC60). However, little is known about its biogeochemical cycling in natural waters. In this paper, the interactions between nC60 and fulvic acids were investigated using fluorescence spectroscopy and fluorescence quenching titration. The results show that the intrinsic fluorescence of fulvic acids was static quenched by adding nC60. The association constants (log K) of fulvic acids and nC60 were estimated using a modified Ryan–Weber nonlinear model, and ranged from 6.76 to 7.41 l/mol. The log K was not significantly affected by the concentration levels of fulvic acids from 5.0 to 20.0 mg/l. The log K increased at low pH 3–5, but remained constant at high pH ranging from 5 to 11. The hydrophobic and π–π interactions were the likely primary mechanisms. The present observation will be helpful in understanding the environmental behavior of fullerene in natural aquatic ecosystems. ► Fluorescence of fulvic acids can be quenched by nanoscaled-fullerene-aggregates. ► Static quenching was the main fluorescence quenching mechanism. ► Association constants were estimated with fluorescence quenching titration. ► Hydrophobic and π–π interactions control the interaction. Interactions between nanoscaled-fullerene-aggregates and fulvic acids in water were quantitatively investigated by fluorescence quenching titration.