In vitro Influence of Phenylalanine on Acetylcholinesterase Activity and DNA

Acetylcholinesterase (AChE) is a significant compo­nent of the membrane contributing to the permeability changes during synaptic transmission and conduction. Phenylketonuria is a group of metabolic disorders in which phenylalanine (Phe) is highly elevated in blood (up to 0 .1 ᴍ) resulting in*mental...

Full description

Saved in:
Bibliographic Details
Published inZeitschrift für Naturforschung C. A journal of biosciences Vol. 53; no. 3; pp. 291 - 293
Main Authors Schulpis, Kleopatra H., Karikas, George A., Tjamouranis, Joana
Format Journal Article
LanguageEnglish
Published Germany Verlag der Zeitschrift für Naturforschung 01.04.1998
Subjects
Acetylcholinesterase
Acetylcholinesterase - metabolism
Animals
Cattle
DNA
DNA - chemistry
DNA - drug effects
Doxorubicin - pharmacology
Humans
Kinetics
Phenylalanine
Phenylalanine - pharmacology
Thymus Gland
Online AccessGet full text

Cover

More Information
Summary:Acetylcholinesterase (AChE) is a significant compo­nent of the membrane contributing to the permeability changes during synaptic transmission and conduction. Phenylketonuria is a group of metabolic disorders in which phenylalanine (Phe) is highly elevated in blood (up to 0 .1 ᴍ) resulting in*mental retardation etc. AChE activity was measured spectrophotometrically after incu­ bation with various Phe concentrations. Phe interaction with DNA was evaluated with an established HPLC method. Phe was found to inhibit AChE almost 40% , at a concentration of 5 mᴍ, whereas a 62.5% DNA peak exclusion (molecular interaction) was observed when Phe was incubated with DNA at a concentration of 3 mᴍ. In addition the ratio of DNA: Phe determined the potency of the observed molecular effect.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0939-5075
1865-7125
DOI:10.1515/znc-1998-3-422