Contribution of plasma, placental, inflammatory and pro-resolving mediators in labor induction

• Published in: Placenta (Eastbourne) Vol. 122; pp. 9 - 17
• Main Authors: Volpato, Lia Karina, Nadkarni, Suchita, Horevicz, Veronica Vargas, Donatello, Nathalia, Cremona Parma, Gabriel Oscar, Martins, Daniel Fernandes, Piovezan, Anna Paula
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.05.2022
• Subjects: Inflammation; Labor; Pregnancy; Resolution of inflammation; Pregnancy; Inflammation; Resolution of inflammation; Labor
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/j.placenta.2022.03.014

The onset of labor is regulated by endocrine, nervous and immunological factors. This study was designed to determine the inflammatory and pro-resolving mediator levels in plasma and placenta of women undergoing labor induction in late-term pregnancy. Healthy pregnant women admitted for delivery or labor induction were included. TNF, IL-1β, IL-6, IL-8, and IL-10 were quantified by ELISA in plasmatic and placental samples and Annexin A1 (ANXA1) in the placenta by Western Blotting, and immunofluorescence to CD15+ antibody. The data were analyzed using the Wilcoxon test and Spearman correlation. The p-value was significant when <0.05. There was a higher concentration of IL-8 was found in the amniotic plaque (p = 0.042) and IL-10 (p < 0.001) in the trophoblast of patients with spontaneous labor. Greater ANXA1 density in the trophoblast was also observed in those with induction failure. There was a positive correlation of ANXA1 density in trophoblast induction duration with (r = 0.580) and with the IL-6 level in amniotic plaque (r = 0.517), and a positive correlation between labor duration and density of ANXA1 was identified in the trophoblast (r = 0.419). An increase was identified in CD15+ cell immunocapturing among the groups with spontaneous labor compared to the group with induction failure (p < 0.001). The inflammatory process in labor involves both maternal and fetal participation. Induction failure is associated with higher levels of ANXA1 in the trophoblast. •Higher concentration of IL-8, IL-10 in spontaneous labor.•Lower density of ANXA1 in patients subjected to induction with good response.•Positive correlations between induction time with IL-6 and ANXA1.•Positive correlations between labor time and ANXA1.