Delineation of a 150-kb breakpoint cluster in benign thyroid tumors with 19q13.4 aberrations

• Published in: Cytogenetic and genome research Vol. 93; no. 1-2; pp. 48 - 51
• Main Authors: Belge, G., Rippe, V., Meiboom, M., Drieschner, N., Garcia, E., Bullerdiek, J.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.2001; S. Karger AG
• Subjects: Adenoma - genetics; Biological and medical sciences; chromosome 19; Chromosome Banding; Chromosome Breakage - genetics; Chromosomes, Human, Pair 19 - genetics; Classical genetics, quantitative genetics, hybrids; Fibroblasts; Fundamental and applied biological sciences. Psychology; Gene Mapping, Cloning and Sequencing; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; In Situ Hybridization, Fluorescence; Physical Chromosome Mapping; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Tagged Sites; Thyroid Neoplasms - genetics; Translocation, Genetic - genetics; Tumor Cells, Cultured; Chromosomal aberration; Genetic mapping; Chromosome F19; Human; Breakpoint; Thyroid diseases; Abnormal chromosome; Thyroid gland; Benign neoplasm; Sequence tagged site; Physical map; Chromosome translocation
• ISSN: 1424-8581; 0301-0171; 1424-859X
• DOI: 10.1159/000056947

Structural rearrangements involving the long arm of chromosome 19 characterize a cytogenetic subgroup of benign thyroid tumors and constitute one of the most frequent specific chromosome abnormalities in epithelial tumors. Recently, we have been able to narrow down the breakpoint region affected in two cell lines to a region covered by a single PAC clone. Close to that region a candidate gene has been identified which we tentatively referred to as RITA (Rearranged In Thyroid Adenomas) now named ZNF331 according to HUGO nomenclature. However, the results had been obtained on two cell lines only making it necessary to extend the studies to a larger number of tumors including primary material. Herein, we have used four further primary tumors showing translocations involving 19q13 for fluorescence in situ hybridization (FISH) mapping studies using a variety of molecular probes from a 470-kbp cosmid/BAC contig. Ten new STSs were characterized and physically mapped within an EcoRI restriction map. The results enabled us to define an approximately 150-kbp breakpoint cluster region of the 19q13 aberrations in benign thyroid tumors flanked by two newly established STS markers.