Effects of surface charge and ligand type of Au nanoparticles on green fluorescent protein-expressing Escherichia coli

• Published in: Biotechnology and bioprocess engineering Vol. 22; no. 1; pp. 83 - 88
• Main Authors: Park, Jeong Chan, Lee, Gyeong Tae, Kim, Chang Sup, Seo, Jeong Hyun
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.02.2017; Springer Nature B.V; 한국생물공학회
• Subjects: absorbance; Analysis; Bacteria; Biotechnology; Cell growth; Chemical engineering; Chemistry; Chemistry and Materials Science; citrates; E coli; Escherichia coli; Fluorescence; gold; green fluorescent protein; Industrial and Production Engineering; Ligands; Metabolism; Microorganisms; monitoring; nanogold; Nanomaterials; Nanoparticles; Nanotechnology; Optical properties; Polyethylene glycol; polyethyleneimine; Protein expression; Proteins; Research Paper; Studies; Toxicity; Transmission electron microscopy; 생물공학; Au nanoparticle; surface charge; ligand type; optical density; fluorescence intensity
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-016-0595-5

The effects of the surface charge and ligand type of three types of Au nanoparticles (NPs), namely anionic polyethylene glycol (PEG)-Au NPs, anionic citrate (Cit)-Au NPs, and cationic branched polyethylenimine (bPEI)-Au NPs, on green fluorescent protein (GFP)-expressing Escherichia coli were evaluated through the combined analysis of optical density (OD) and fluorescence intensity (FI). OD and FI can provide information about cell growth and metabolism of bacteria, respectively. The results demonstrated that PEG- and Cit-Au NPs had no major effects on the OD and FI of GFP-expressing bacteria. However, it was found that Cit-Au NPs may slightly influence cell metabolism at higher concentrations, although it is necessary to perform further in-depth study to clarify this issue. Cationic bPEI-Au NPs showed significant effects on cell density and metabolism of E. coli , with an especially strong effect on metabolism. The combined analysis of OD and FI may be useful for monitoring the effects of a wide range of nanomaterials on microorganisms.