Prenatal BACs-on-Beads™: a new technology for rapid detection of aneuploidies and microdeletions in prenatal diagnosis

• Published in: Prenatal diagnosis Vol. 31; no. 5; pp. 500 - 508
• Main Authors: Vialard, F., Simoni, G., Aboura, A., De Toffol, S., Molina Gomes, D., Marcato, L., Serero, S., Clement, P., Bouhanna, P., Rouleau, E., Grimi, B., Selva, J., Gaetani, E., Maggi, F., Joseph, A., Benzacken, B., Grati, F. R.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.05.2011; Wiley
• Subjects: Adult; Aneuploidy; BACs-on-Beads; Biological and medical sciences; Chorionic Villi Sampling; Chromosomes, Artificial, Bacterial - genetics; Cordocentesis; Delivery. Postpartum. Lactation; DNA - analysis; Female; Fetal Blood; Fundamental and applied biological sciences. Psychology; Gene Deletion; Genetic Diseases, Inborn - diagnosis; Genetic Diseases, Inborn - genetics; Genetics of eukaryotes. Biological and molecular evolution; Gynecology. Andrology. Obstetrics; Humans; In Situ Hybridization, Fluorescence; Male; Medical sciences; microdeletions; Molecular and cellular biology; Mosaicism; Predictive Value of Tests; prenatal diagnosis; Prenatal Diagnosis - economics; Prenatal Diagnosis - methods; Prospective Studies; Retrospective Studies; screening; Chromosomal aberration; screening; Gynecology; Neonatology; Aneuploidy; Medical screening; microdeletions; Prenatal; Rapid technique; Prenatal diagnosis; BACs-on-Beads; Technology; Genetics
• ISSN: 0197-3851; 1097-0223; 1097-0223
• DOI: 10.1002/pd.2727

Objective Molecular cytogenetic techniques on uncultured prenatal samples are the sole tests applied in some countries in cases with advanced maternal age (AMA) or increased risk after prenatal screening. Moreover, there is a trend to perform invasive prenatal diagnosis (PD) during the first trimester before ultrasound manifestations, so new rapid and reliable assays are necessary to investigate microdeletions not detectable with the conventional karyotype. We report the validation study of the prenatal bacterial artificial chromosomes‐on‐Beads™ (BoBs™; CE‐IVD), a bead‐based multiplex assay detecting chromosomes 13, 18, 21, X/Y aneuploidies and nine microdeletion regions having an overall detection rate of 1/1700. Method We retrospectively studied 408 selected samples and prospectively tested 212 consecutive samples ascertained for conventional karyotyping. Results We did not find false‐positive results. Triploidies were not detected. Maternal cell contamination of male samples up to 90% was unmasked inspecting gonosome profiles. Mosaic conditions at 20 to 30% were revealed. Failures were due to low amount of DNA. Conclusion Prenatal BoBs™ is a robust technology for the investigation of fetuses with normal karyotype with or without sonographic abnormalities. Running in parallel with the karyotype analysis, it can be proposed instead of rapid FISH or QF‐PCR providing rapid results on common aneuploidies and additional information regarding the microdeletion syndromes. Copyright © 2011 John Wiley & Sons, Ltd.