Molecular epidemiology of rotaviral infection in South Indian children with acute diarrhea from 1995-1996 to 1998-1999

• Published in: Journal of medical virology Vol. 67; no. 1; pp. 101 - 105
• Main Authors: Kang, Gagandeep, Green, Jonathan, Gallimore, Christopher I., Brown, David W.G.
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 01.05.2002; Wiley-Liss
• Subjects: Acute Disease; Animals; Antigens, Viral; Biological and medical sciences; Capsid - genetics; Capsid Proteins; Cattle; Child, Preschool; Diarrhea - epidemiology; Diarrhea - physiopathology; Diarrhea - virology; DNA, Viral - analysis; Epidemiology; Fundamental and applied biological sciences. Psychology; Genotype; genotypes; Human viral diseases; Humans; India; India - epidemiology; Infectious diseases; Medical sciences; Microbiology; Molecular Epidemiology; RNA, Viral - analysis; rotavirus; Rotavirus - classification; Rotavirus - genetics; Rotavirus Infections - epidemiology; Rotavirus Infections - physiopathology; Rotavirus Infections - virology; Sequence Analysis, DNA; Viral diseases; Viral diseases of the digestive system; Virology; Asia; India; Human; Rotavirus; Human rotavirus; Acute; Gastroenteritis; Diarrhea; Genotype; Reoviridae; Infection; Virus; Viral disease; Molecular epidemiology; Digestive diseases; Intestinal disease; Child; Gastric disease
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.2197

The distribution of VP7 (G‐) and VP4 (P‐) genotypes among 126 rotavirus strains from South Indian children, < 5 years of age and with acute diarrhoea, presenting to a single hospital during the months to November and December, from 1995 to 1998, was studied. Multiplex hemi‐nested G‐ and P‐typing polymerase chain reactions determined 101 (80%) G types and 78 (61%) P types, respectively. In order of frequency, the commonest G types were G1, G4, G2, G9, G3, and G8, and P types were P1B[4], P1A[8], and P2A[6] and the most common G:P combinations were G1:P1A[8], G1:P1B[4], G2P1B[4] and G4:P1A[8]. G1, G2, and G4 types were seen in all years. The single G3 isolate was seen in 1998. The single G8 isolate and the 5 G9 isolates were seen in 1997, after a period of heavy rain. Sequence analysis showed that the G8 isolate was related most closely to the bovine strain A5, and the G9 strains were distinct from the nonpathogenic Indian isolate 116E and similar to G9s isolated in Mysore and the United Kingdom described previously. J. Med. Virol. 67:101–105, 2002. © 2002 Wiley‐Liss, Inc.