The expression of LytM is down-regulated by RNAIII in Staphylococcus aureus

• Published in: Journal of basic microbiology Vol. 52; no. 6; pp. 636 - 641
• Main Authors: Chunhua, Mu, Yu, Liu, Yaping, Gao, Jie, Dong, Qiang, Lu, Xiaorong, Tan, Guang, Yang
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.12.2012; WILEY‐VCH Verlag
• Subjects: 5' Untranslated Regions; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Binding Sites; Down-Regulation; Endopeptidases - biosynthesis; Endopeptidases - genetics; Endopeptidases - metabolism; LytM; N-Acetylmuramoyl-L-alanine Amidase - genetics; N-Acetylmuramoyl-L-alanine Amidase - metabolism; Ribosomes - genetics; Ribosomes - metabolism; RNA III; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Messenger - genetics; S. aureus; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus aureus - metabolism
• ISSN: 0233-111X; 1521-4028
• DOI: 10.1002/jobm.201100426

LytM is one kind of autolysin expressed in Staphylococcus aureus. It can hydrolyze peptide bond of pentaglycine, which is the component of cell wall. Until now, the regulation on the expression of LytM is still unclear. Here, we found that the level of LytM in the RNAIII mutant was increased. RNAIII is an important small regulatory RNA in S. aureus. Our further investigation revealed that RNAIII can interact with the 5′UTR of lytM mRNA and block the ribosome binding sites [RBS]. So our results identified that LytM was the new target of RNAIII. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)