Structural characterization and biological activity of an α-glucan from the mollusk Marcia hiantina (Lamarck, 1818)

• Published in: Glycoconjugate journal Vol. 40; no. 1; pp. 33 - 46
• Main Authors: Ahmed, Hoda Al, Ticar, Bernadeth F., Black, Ian, Mahdi, Fakhri, Shami, Anter A., Misra, Sandeep K., Heiss, Christian, Paris, Jason J., Sharp, Joshua S., Azadi, Parastoo, Pomin, Vitor H.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2023; Springer Nature B.V; Springer
• Subjects: Animals; BASIC BIOLOGICAL SCIENCES; Biochemistry; Biological activity; Biomedical and Life Sciences; bivalve; Bivalvia; Cell lines; Cell proliferation; Chromatography; Chromatography, Gel; Coastal zone; Cytotoxicity; Gas chromatography; glucan; Glucans - chemistry; Hydrogen Peroxide; Immunomodulation; Ions; Life Sciences; Light scattering; Lipopolysaccharides; Marcia hiantina; Mass spectroscopy; Methylation; methylation analysis; Molecular weight; mollusk; Monosaccharides; NMR; Nuclear magnetic resonance; Original Article; Pathology; Polysaccharides; Polysaccharides - chemistry; Reactive Oxygen Species; Glucan; NMR; Mollusk; Bivalve; Methylation analysis; Marcia hiantina
• ISSN: 0282-0080; 1573-4986; 1573-4986
• DOI: 10.1007/s10719-022-10092-6

Marcia hiantina (Mollusca, Bivalvia) (Lamarck, 1818), is an edible clam mainly distributed along the tropical coastal regions. Recent researches have demonstrated that clams can possess compounds, including polysaccharides, with a wide range of biological actions including antioxidant, immunomodulatory and antitumor activities. Here an α-glucan was isolated from M. hiantina by hot water, purified by anion exchange chromatography, and its structure was characterized by a combination of multiple nuclear magnetic resonance (NMR) methods (1D 1 H, 1 H- 1 H COSY, 1 H- 1 H TOCSY, 1 H- 1 H NOESY, 1 H- 13 C HSQC and 1 H- 13 C HSQC-NOESY spectra), gas chromatography-mass spectrometry, and high performance size exclusion chromatography (HPSEC). The analysis from NMR, monosaccharide composition, methylation analyses and HPSEC combined with multi-angle light scattering (MALS) of M. hiantina -derived α-glycan confirmed a branched polysaccharide exclusively composed of glucose (Glc), mostly 4-linked in its backbone, branched occasionally at 6-positions, and having a molecular weight of ~ 570 kDa. The mollusk α-glucan was subjected to four cell-based assays: (i) viability of three cell lines (RAW264.7, HaCaT, and HT-29), (ii) activity on lipopolysaccharide (LPS)-induced prostaglandin production in RAW264.7 cells, (iii) inhibitory activities of in H 2 O 2 - and LPS-induced reactive oxygen species (ROS) production in HMC3 cells, and (iv) HaCaT cell proliferation. Results have indicated no cytotoxicity, potent inhibition of both H 2 O 2 - and LPS-induced ROS, and potent cell proliferative activity.