Circulating endothelial precursor cells are associated with a healed diabetic foot ulcer evaluated in a prospective cohort study

• Published in: Wound repair and regeneration Vol. 31; no. 1; pp. 128 - 134
• Main Authors: Margolis, David J., Mitra, Nandita, Hoffstad, Ole, Malay, D. Scot, Mirza, Ziad K., Lantis, John C., Lev‐Tov, Hadar A., Kirsner, Robert S., Ruhela, Deepa, Bhopale, Veena M., Thom, Stephan R.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.01.2023
• Subjects: biomarker; Diabetes Mellitus; Diabetic Foot; diabetic foot ulcer; endothelial precursor cells; Humans; Prognosis; Prospective Studies; Wound Healing; biomarker; diabetic foot ulcer; endothelial precursor cells
• ISSN: 1067-1927; 1524-475X
• DOI: 10.1111/wrr.13055

The goal of this multicentre study was to evaluate whether circulating endothelial precursor cells and microparticles can predict diabetic foot ulcer healing by the 16th week of care. We enrolled 207 subjects, and 40.0% (28.4, 41.5) healed by the 16th week of care. Using flow cytometry analysis, several circulating endothelial precursor cells measured at the first week of care were associated with healing after adjustment for wound area and wound duration. For example, CD34+CD45dim, the univariate odds ratio was 1.19 (95% confidence interval: 0.88, 1.61) and after adjustment for wound area and wound duration, the odds ratio was (1.67 (1.16, 2.42) p = 0.006). A prognostic model using CD34+ CD45dim, wound area, and wound duration had an area under the curve of 0.75 (0.67, 0.82) and CD34+ CD45dim per initial wound area, an area under the curve of 0.72 (0.64, 0.79). Microparticles were not associated with a healed wound. Previous studies have indicated that circulating endothelial precursor cells measured at the first office visit are associated with a healed diabetic foot ulcer. In this multicentred prospective study, we confirm this finding, show the importance of adjusting circulating endothelial precursor cells measurements by wound area, and show circulating endothelial precursor cells per wound area is highly predictive of a healed diabetic foot ulcer by 16th week of care.