Validation of HPLC method for the simultaneous and quantitative determination of 12 UV-filters in cosmetics

• Published in: International journal of cosmetic science Vol. 32; no. 1; pp. 47 - 53
• Main Authors: Nyeborg, M., Pissavini, M., Lemasson, Y., Doucet, O.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2010; Wiley
• Subjects: Analytical chemistry; Applied sciences; Chemical industry and chemicals; Chemistry; Chemistry, Pharmaceutical; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; cosmetics; Cosmetics - chemistry; Cosmetics, toiletries; Exact sciences and technology; HPLC; Humans; Other chromatographic methods; Spectrophotometry, Ultraviolet; Sunscreening Agents - analysis; sunscreens; UV-filters; validation; Washing products. Cosmetics and toiletries. Perfumes; Cosmetic; Validation; Chemical analysis; sunscreens; HPLC chromatography; UV-filters; cosmetics; Reversed phase chromatography; Ultraviolet detector; Sunscreen product; Ultraviolet filter; HPLC; Quantitative analysis
• ISSN: 0142-5463; 1468-2494
• DOI: 10.1111/j.1468-2494.2009.00544.x

Synopsis The aim of the study was the validation of a high‐performance liquid chromatography (HPLC) method for the simultaneous and quantitative determination of twelve commonly used organic UV‐filters (phenylbenzimidazole sulfonic acid, benzophenone‐3, isoamyl p‐methoxycinnamate, diethylamino hydroxybenzoyl hexyl benzoate, octocrylene, ethylhexyl methoxycinnamate, ethylhexyl salicylate, butyl methoxydibenzoylmethane, diethylhexyl butamido triazone, ethylhexyl triazone, methylene bis‐benzotriazolyl tetramethylbutylphenol and bis‐ethylhexyloxyphenol methoxyphenyl triazine) contained in suncare products. The separation and quantitative determination was performed in <30 min, using a Symmetry Shield® C18 (5 μm) column from Waters and a mobile phase (gradient mode) consisting of ethanol and acidified water. UV measurements were carried out at multi‐wavelengths, according to the absorption of the analytes. Résumé L’objectif de cette étude a été de valider une méthode de chromatographie liquide haute performance (CLHP) pour la quantification simultanée de douze filtres UV organiques communément utilisés (phenylbenzimidazole sulfonic acid, benzophenone‐3, isoamyl p‐methoxycinnamate, diethylamino hydroxylbenzoyl hexyl benzoate, octocrylene, ethylhexyl methoxycinnamate, ethylhexyl salicylate, butyl methoxydibenzoylmethane, diethylhexyl butamido triazone, ethylhexyl triazone, methylene bis‐benzotriazolyl tetramethylbutylphenol et bis‐ethylhexyloxyphenol methoxyphenyl triazine) contenus dans les produits solaires. La séparation et la quantification ont été réalisées en moins de 30 min, en utilisant une colonne Symmetry Shield® C18 (5 μm) Waters et une phase mobile (mode gradient) composée d’éthanol et d’eau acidifiée. Les mesures ont été effectuées sous plusieurs longueurs d’onde, selon le degré d’absorption des analytes.