Influence of IL-6, IL-8, and TGF-β1 gene polymorphisms on the risk of human papillomavirus-infection in women from Pernambuco, Brazil

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 111; no. 11; pp. 663 - 669
• Main Authors: Lima, Júnior, Sérgio Ferreira de, Tavares, Mayara Mansur Fernandes, Macedo, Jamilly Lopes de, Oliveira, Renata Santos de, Heráclio, Sandra de Andrade, Maia, Maria de Mascena Diniz, Souza, Paulo Roberto Eleutério de, Moura, Ronald, Crovella, Sergio
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01.11.2016; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Adolescent; Adult; Aged; Alleles; Base Sequence; Brazil; Cervical Intraepithelial Neoplasia - genetics; Cervical Intraepithelial Neoplasia - virology; Cross-Sectional Studies; cytokine; DNA, Viral - analysis; Female; Gene Frequency; Genetic Predisposition to Disease; host genome; HPV; Humans; Interleukin-6 - genetics; Interleukin-8 - genetics; Middle Aged; Papillomavirus Infections - genetics; Papillomavirus Infections - virology; PARASITOLOGY; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; polymorphisms; Transforming Growth Factor beta1 - genetics; TROPICAL MEDICINE; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - virology; Young Adult; cytokine; host genome; polymorphisms; HPV
• ISSN: 0074-0276; 1678-8060; 1678-8060
• DOI: 10.1590/0074-02760160051

Human papillomavirus (HPV) infections are strongly associated with the development of cervical intraepithelial neoplasias and invasive cervical cancer. Polymorphisms in cytokine-encoding genes and behavioural cofactors could play an important role in protecting an individual against viral infections and cancer. Here, we investigated whether IL-6 -174 G>C, IL-8 +396 G>T, and TGF-β1 +869 G>C and +915 G>C polymorphisms were associated with susceptibility to HPV infection in women from north-east (Pernambuco) Brazil. We analysed 108 healthy uninfected women (HC) and 108 HPV-positive women with cervical lesions. Genetic polymorphisms were assessed using Sanger sequencing and polymerase chain reaction-restriction fragment length polymorphism. Comparison of the distribution of the genotypic and allelic frequencies of the IL-18 +396 T>G polymorphism between HPV infected woman an uninfected controls showed that the GG genotype and G allele were both more frequent in the HC group, and were associated with protection from HPV infection (p = 0.0015; OR = 0.29 CI95% = 0.13-0.61; p = 0.0005; OR = 0.45 CI95% 0.29-0.7, respectively). Individuals from the control group could have previously had HPV infection that was spontaneously eliminated; however, it was undetectable at the time of sample collection. Based on our findings, we hypothesize that the IL-8 +396 G>T polymorphism could interfere with susceptibility to HPV infection, by modulating the ability of immune system to fight the virus.