Selected ion monitoring/isotope dilution mass spectrometric determination of 1-aminocyclopropane-1-carboxylic acid levels in ripening tomato fruit

A new method is described for the quantitation of 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene in plants. [2,2,3,3,- 2H 4]ACC has been synthesized and used as an internal standard for selected ion monitoring/isotope dilution quantitation of this compound in ripeni...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 149; no. 1; pp. 130 - 135
Main Authors McGaw, Brian A., Horgan, Roger, Heald, Jim K.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.08.1985
Elsevier
Subjects
ACC
Amino Acids - analysis
Amino Acids, Cyclic
Analysis of complex biological substances
Analytical, structural and metabolic biochemistry
Applied sciences
Biological and medical sciences
Carotenoids - analysis
Chromatography, High Pressure Liquid
Ethylenes - analysis
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry - methods
Lycopene
Other techniques and industries
Oxidation-Reduction
Plants and fungi
quantitative mass spectrometry
tomato fruit
Vegetables - analysis
quantitative mass spectrometry
ACC
tomato fruit
Assay
Ripening
Gas chromatography
Dicotyledones
Angiospermae
Ethylene
Lycopersicon esculentum
Spermatophyta
Biosynthesis precursor
Solanaceae
Mass spectrometry
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Summary:A new method is described for the quantitation of 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene in plants. [2,2,3,3,- 2H 4]ACC has been synthesized and used as an internal standard for selected ion monitoring/isotope dilution quantitation of this compound in ripening tomato fruit. These data are compared with those derived from the widely used indirect oxidative ACC assay (which underestimated the ACC levels by between two- and fourfold). The greater accuracy, sensitivity (100X), and specificity of the mass spectrometric method will be of considerable benefit to those interested in factors which control ACC and ultimately ethylene levels since it is believed that ACC synthesis and its oxidative metabolism to ethylene are the key points at which ethylene biosynthesis is regulated.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(85)90485-3