Generation of hepatocytes expressing functional cytochromes P450 from a pancreatic progenitor cell line in vitro

The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimul...

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Bibliographic Details
Published inBiochemical journal Vol. 370; no. Pt 3; pp. 763 - 769
Main Authors Marek, Carylyn J, Cameron, Gary A, Elrick, Lucy J, Hawksworth, Gabrielle M, Wright, Matthew C
Format Journal Article
LanguageEnglish
Published England 15.03.2003
Subjects
Animals
Cell Differentiation
Cell Line
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - metabolism
Dexamethasone - pharmacology
Glucocorticoids - pharmacology
Hepatocytes - cytology
Hepatocytes - metabolism
Male
Pancreas - cytology
Phenotype
Rats
Rats, Sprague-Dawley
Testosterone - metabolism
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Summary:The proliferating AR42J-B13 pancreatic cell line is known to respond to glucocorticoid treatment by producing foci of cells that express the liver-specific albumin gene. We demonstrate that this cell line also expresses liver-specific or liver-enriched functional cytochrome P450 proteins when stimulated to trans-differentiate into hepatocytes by glucocorticoid. These data suggest that this cell line has an unusual ability to trans-differentiate into functional hepatocytes and that it could be possible to generate a limitless supply of functional hepatocyte-like cells in vitro.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0264-6021
1470-8728
DOI:10.1042/BJ20021545