Diversity of Type II restriction endonucleases that require two DNA recognition sites
Orthodox Type IIP restriction endonucleases, which are commonly used in molecular biological work, recognize a single palindromic DNA recognition sequence and cleave within or near this sequence. Several new studies have reported on structural and biochemical peculiarities of restriction endonucleas...
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Published in | Nucleic acids research Vol. 31; no. 21; pp. 6079 - 6084 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.11.2003
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | Orthodox Type IIP restriction endonucleases, which are commonly used in molecular biological work, recognize a single palindromic DNA recognition sequence and cleave within or near this sequence. Several new studies have reported on structural and biochemical peculiarities of restriction endonucleases that differ from the orthodox in that they require two copies of a particular DNA recognition sequence to cleave the DNA. These two sites requiring restriction endonucleases belong to different subtypes of Type II restriction endonucleases, namely Types IIE, IIF and IIS. We compare enzymes of these three types with regard to their DNA recognition and cleavage properties. The simultaneous recognition of two identical DNA sites by these restriction endonucleases ensures that single unmethylated recognition sites do not lead to chromosomal DNA cleavage, and might reflect evolutionary connections to other DNA processing proteins that specifically function with two sites. |
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Bibliography: | local:gkg836 Received July 3, 2003; Revised and Accepted September 16, 2003 istex:1C912CFD6936C06EF9642A59407197FFA2BEB022 To whom correspondence should be addressed. Tel: +49 30 450 525 092; Fax: +49 30 450 525 907; Email: detlev.kruger@charite.de Present address: Merlind Mucke, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA ark:/67375/HXZ-85BWC83Q-8 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0305-1048 1362-4962 1362-4962 |
DOI: | 10.1093/nar/gkg836 |