Sensitive and Stable Molecular Detection of Dengue, Chikungunya, and Zika Viruses from Dried Blood Spots

Standard molecular detection of many pathogens, in particular RNA viruses, requires appropriate handling in the field for preserving the quality of the sample until processing. This could be challenging in remote tropical areas. Dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) a...

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Published inThe American journal of tropical medicine and hygiene Vol. 107; no. 2; pp. 296 - 299
Main Authors Cardona-Ospina, Jaime Andrés, Stittleburg, Victoria, Millan-Benavidez, Natalia, Restrepo-Chica, Juliana, Key, Autum, Rojas-Gallardo, Diana Marcela, Piantadosi, Anne, Collins, Matthew H, Waggoner, Jesse J
Format Journal Article
LanguageEnglish
Published United States Institute of Tropical Medicine 01.08.2022
The American Society of Tropical Medicine and Hygiene
Subjects
Blood
Chikungunya virus
Dengue fever
Diagnostic tests
Parasitic diseases
Ribonucleic acid
RNA
Short Report
Zika virus
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Summary:Standard molecular detection of many pathogens, in particular RNA viruses, requires appropriate handling in the field for preserving the quality of the sample until processing. This could be challenging in remote tropical areas. Dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) are RNA viruses, prominent among the causes of fever in the tropics. We aimed to test the stability of arboviral RNA in contrived dried blood spots prepared on Whatman 903 Protein saver cards as a means of sample collection and storage. We were able to detect DENV, CHIKV, and ZIKV by real-time RT-PCR up to 180 days after card inoculation with stable Ct values across the study period. Our study supports dried blood spots (DBS) on protein saver cards as a platform for stable detection of arboviral RNA of sufficient quality to be used in diagnostic RT-PCR assays and next generation sequencing.
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Financial support: This research was supported in part by the Halle Institute for Global Research and the University Research Committee of Emory University, and by the Ministerio de Ciencia, Tecnología e Innovación de Colombia, (Minciencias), Contract No 419-2020.
Authors’ addresses: Jaime A. Cardona-Ospina, Grupo de Investigación Biomedicina, Facultad de Medicina, Institución Universitaria Visión de las Américas, Pereira, Risaralda, Colombia, and Emerging Infectious Diseases and Tropical Medicine Research Group, Sci-help, Pereira, Risaralda, Colombia, E-mail: jaime.cardona@uam.edu.co. Victoria Stittleburg, Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, GA, E-mail: victoria.d.simmons@emory.edu. Natalia Millan-Benavidez, Juliana Restrepo-Chica, and Diana Marcela Rojas-Gallardo, Grupo de Investigación Biomedicina, Facultad de Medicina, Institución Universitaria Visión de las Américas, Pereira, Risaralda, Colombia, E-mails: natalia.millan@uam.edu.co, juliana.restrepoc@uam.edu.co, and diana.rojasg@uam.edu.co. Autum Key, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, E-mail: autum.key@emory.edu. Anne Piantadosi, Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, GA, and Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, E-mail: anne.piantadosi@emory.edu. Matthew H. Collins and Jesse J. Waggoner, Department of Medicine, Division of Infectious Diseases, Emory University, Atlanta, GA, and Department of Global Health, Rollins School of Public Health, Atlanta, GA, Emails: matthew.collins@emory.edu and jjwaggo@emory.edu.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.21-1087