Mutational signatures of DNA mismatch repair deficiency in C. elegans and human cancers
Throughout their lifetime, cells are subject to extrinsic and intrinsic mutational processes leaving behind characteristic signatures in the genome. DNA mismatch repair (MMR) deficiency leads to hypermutation and is found in different cancer types. Although it is possible to associate mutational sig...
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Published in | Genome research Vol. 28; no. 5; pp. 666 - 675 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Cold Spring Harbor Laboratory Press
01.05.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Throughout their lifetime, cells are subject to extrinsic and intrinsic mutational processes leaving behind characteristic signatures in the genome. DNA mismatch repair (MMR) deficiency leads to hypermutation and is found in different cancer types. Although it is possible to associate mutational signatures extracted from human cancers with possible mutational processes, the exact causation is often unknown. Here, we use
C. elegans
genome sequencing of
pms-2
and
mlh-1
knockouts to reveal the mutational patterns linked to
C. elegans
MMR deficiency and their dependency on endogenous replication errors and errors caused by deletion of the polymerase ε subunit
pole-4
. Signature extraction from 215 human colorectal and 289 gastric adenocarcinomas revealed three MMR-associated signatures, one of which closely resembles the
C. elegans
MMR spectrum and strongly discriminates microsatellite stable and unstable tumors (AUC = 98%). A characteristic difference between human and
C. elegans
MMR deficiency is the lack of elevated levels of N
C
G > NTG mutations in
C. elegans,
likely caused by the absence of cytosine (CpG) methylation in worms
.
The other two human MMR signatures may reflect the interaction between MMR deficiency and other mutagenic processes, but their exact cause remains unknown. In summary, combining information from genetically defined models and cancer samples allows for better aligning mutational signatures to causal mutagenic processes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work. |
ISSN: | 1088-9051 1549-5469 1549-5469 |
DOI: | 10.1101/gr.226845.117 |