Microsatellite loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and transferability to other Lepidieae

Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci i...

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Published inInternational journal of molecular sciences Vol. 13; no. 9; pp. 11861 - 11869
Main Authors Martínez-Nieto, María Isabel, Merlo, María Encarna, Mota, Juan Francisco, Salmerón-Sánchez, Esteban, Segarra-Moragues, José Gabriel
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.09.2012
Molecular Diversity Preservation International (MDPI)
Subjects
Biodiversity
Gene Transfer, Horizontal
Genetic diversity
Genetic Loci
Habitats
Investigations
Lepidium - classification
Lepidium - genetics
Microsatellite Repeats
Phylogeny
Polymorphism, Genetic
Population genetics
Predation
Spain
genetic diversity
gypsophile
SSR
Brassicaceae
next-generation sequencing
conservation genetics
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Summary:Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci including di- and tri-nucleotide repeats were characterized in L. subulatum. They amplified a total of 80 alleles (2-12 alleles per locus) in a sample of 35 individuals of L. subulatum, showing relatively high levels of genetic diversity, H(O) = 0.645, H(E) = 0.627. Cross-species transferability of all 12 loci was successful for the Iberian endemics Lepidium cardamines, Lepidium stylatum, and the widespread, Lepidium graminifolium and one species each of two related genera, Cardaria draba and Coronopus didymus. These microsatellite primers will be useful to investigate genetic diversity, population structure and to address conservation genetics in species of Lepidium.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms130911861