Genome-Wide Association Studies for Milk Somatic Cell Score in Romanian Dairy Cattle

• Published in: Genes Vol. 12; no. 10; p. 1495
• Main Authors: Ilie, Daniela Elena, Mizeranschi, Alexandru Eugeniu, Mihali, Ciprian Valentin, Neamț, Radu Ionel, Goilean, George Vlad, Georgescu, Ovidiu Ionuț, Zaharie, Daniela, Carabaș, Mihai, Huțu, Ioan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.09.2021; MDPI
• Subjects: Animal lactation; Animal welfare; Animals; Cattle; Chromosomes; Dairy cattle; Death-associated protein kinase; Female; Genetic Predisposition to Disease; Genetic variability; Genome-wide association studies; Genome-Wide Association Study; Genomes; Genotype; Interleukin 1; Laboratories; Lactation - genetics; Mastitis; Mastitis, Bovine - genetics; Mastitis, Bovine - pathology; Matrilysin; Milk; Milk - cytology; Milk - metabolism; Milk production; Phenotype; Polymorphism, Single Nucleotide - genetics; Pregnancy; Quality control; Quantitative Trait Loci - genetics; R&D; Research & development; Romania; Single-nucleotide polymorphism; Ubiquitin-Protein Ligases - genetics; Romania; genome-wide association study (GWAS); mastitis; Romanian cattle; somatic cell score
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes12101495

Mastitis is one of the most frequently encountered diseases in dairy cattle, negatively affecting animal welfare and milk production. For this reason, contributions to understanding its genomic architecture are of great interest. Genome-wide association studies (GWAS) have identified multiple loci associated with somatic cell score (SCS) and mastitis in cattle. However, most of the studies have been conducted in different parts of the world on various breeds, and none of the investigations have studied the genetic architecture of mastitis in Romanian dairy cattle breeds up to this point in time. In this study, we report the first GWAS for SCS in dairy cattle breeds from Romania. For GWAS, we used an Axiom Bovine v3 SNP-chip (>63,000 Single Nucleotide Polymorphism -SNPs) and 33,330 records from 690 cows belonging to Romanian Spotted (RS) and Romanian Brown (RB) cattle. The results found one SNP significantly associated with SCS in the RS breed and 40 suggestive SNPs with -log ( ) from 4 to 4.9 for RS and from 4 to 5.4 in RB. From these, 14 markers were located near 12 known genes ( , , , , , , , , , , and in RB cattle, 3 markers were close to , and genes, while one SNP overlapped the gene in RS cattle. Four genes ( , , and ) associated with SCS in this study were previously reported in different studies. The most significant SNP (rs110749552) associated with SCS was located within the gene. In both breeds, the SNPs and position of association signals were distinct among the three parities, denoting that mastitis is controlled by different genes that are dependent according to parity. The current results contribute to an expansion in the body of knowledge regarding the proportion of genetic variability explained by SNPs for SCS in dairy cattle.