Importance of optimal mixtures of EDTA anticoagulant:blood for the preparation of well-stained avian blood smears

1. This paper describes and illustrates the dangers of sub-optimal mixtures of EDTA anticoagulant:blood during the preparation of avian blood smears. 2. Excessive concentrations of EDTA lead to variable staining and make cellular differentiation more difficult. 3. It is recommended, for the use of a...

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Bibliographic Details
Published inBritish poultry science Vol. 34; no. 3; p. 615
Main Authors Robertson, G W, Maxwell, M H
Format Journal Article
LanguageEnglish
Published England 01.07.1993
Subjects
Animals
Blood Cells - cytology
Blood Specimen Collection - methods
Blood Specimen Collection - veterinary
Chickens
Edetic Acid
Female
Staining and Labeling - veterinary
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Summary:1. This paper describes and illustrates the dangers of sub-optimal mixtures of EDTA anticoagulant:blood during the preparation of avian blood smears. 2. Excessive concentrations of EDTA lead to variable staining and make cellular differentiation more difficult. 3. It is recommended, for the use of a 2.5 ml graded blood tube containing 3.75 mg EDTA, that no less than 1.0 ml of blood be mixed.
ISSN:0007-1668
DOI:10.1080/00071669308417617