Methodology for the analysis of fenbendazole and its metabolites in plasma, urine, feces, and tissue homogenates

• Published in: Analytical biochemistry Vol. 155; no. 1; pp. 112 - 118
• Main Authors: Barker, Steven A., Hsieh, Lily C., Short, Charles R.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.05.1986; Elsevier
• Subjects: Analysis; Animals; Applied sciences; Benzimidazoles - analysis; Biological and medical sciences; Chickens; chromatography, general; Chromatography, High Pressure Liquid; drug analysis; drug metabolism; Ducks; Exact sciences and technology; Feces - analysis; Fenbendazole - analysis; Fenbendazole - blood; Fenbendazole - urine; General pharmacology; Goats; HPLC techniques; HPLC, drug metabolites; Liver - analysis; Medical sciences; metabolites, drugs; Other techniques and industries; Pharmacology. Drug treatments; Rabbits; Rats; Species Specificity; HPLC techniques; metabolites, drugs; HPLC, drug metabolites; drug analysis; drug metabolism; chromatography, general; Assay; Biological fluid; Tissue; Anthelmintic; Metabolite; HPLC chromatography; Ultraviolet spectrometry
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(86)90234-4

New methodology for the extraction and analysis of the anthelmintic fenbendazole and its metabolites from plasma, urine, liver homogenates, and feces from several animal species is presented. Quantitation of fenbendazole and its metabolites was conducted by high-pressure liquid chromatography using ultraviolet detection at 290 nm. The combined extraction and analysis procedures give excellent recoveries in all of the different biological matrices examined. High specificity, low limits of detection, and excellent linearity, accuracy, and inter- and intrasample variability were also obtained. The study of fenbendazole pharmacokinetics in vitro and in vivo should be greatly enhanced through the utilization of these methods.