Evaluation of silybum marinaum efficacy on University of Wisconsin and histidine-tryptophan-ketoglutarate solutions latter the damage of the perfused liver

• Published in: Acta cirurgica brasileira Vol. 32; no. 6; pp. 407 - 417
• Main Authors: Özer, Nazmi, Bülbüloğlu, Ertan, Yormaz, Serdar, Ece, İlhan
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01.06.2017
• Subjects: Adenosine; Allopurinol; Animals; Antioxidants; Antioxidants - therapeutic use; Chemical and Drug Induced Liver Injury - drug therapy; Disease Models, Animal; Glucose; Glutathione; Histidine; Immunohistochemistry; Insulin; Liver; Male; Mannitol; Milk Thistle; Organ Preservation Solutions; Potassium Chloride; Procaine; Protective Agents - therapeutic use; Raffinose; Rats; Rats, Wistar; Silymarin - therapeutic use; Tryptophan
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/s0102-865020170060000001

To investigate the hepatoprotective and antioxidant effeicacies of Silybum marianum's (silymarin, S) on University of Wisconsin (UW) and histidinetryptophan-ketoglutarate (HTK) preservation solutions. Thirty two Wistar albino adult male rats were used. Group 1: UW group, Group 2: UW + Silymarin group(S), Group 3: HTK group, Group 4: HTK + silymarin group (S), respectively. Silymarin was enforced intraperitoneally before the surgery. Biopsies were enforced in 0, 6 and 12.hours to investigate. Biochemical parameters examined in alanine aminotransferase (ALT), furthermore superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in rats were also evaluated. Detected histopathological changings were substantially declining in the groups that received silymarin, cellular damage was decreased significantly in HTK + Silymarin group, according to other groups. It has been identified as the most effective group was HTK + silymarin group in evaluation of ALT, electron microscopic results, also decreased MDA and elevated in SOD, and CAT activity. Caspase 3 analysis showed a substantial lower apoptosis ratio in the silymarin groups than in the non-performed groups (p<0.05). Histidinetryptophan-ketoglutarate+silymarin group provides better hepatoprotection than other groups, by decreasing the hepatic pathologic damage, delayed changes that arise under cold ischemic terms.