Bufadienolides and polyhydroxycholestane derivatives from Bufo bufo gargarizans

• Published in: Journal of asian natural products research Vol. 17; no. 4; pp. 364 - 376
• Main Authors: Wang, Yan-Min, Li, Zong-Yun, Wang, Jing-Jing, Wu, Xi-Yan, Gao, Hui-Min, Wang, Zhi-Min
• Format: Journal Article
• Language: English
• Published: ABINGDON Taylor & Francis 03.04.2015; Taylor & Francis Ltd
• Subjects: Animals; bufadienolide; bufadienolides; Bufanolides - chemistry; Bufanolides - isolation & purification; Bufo bufo; Bufo bufo gargarizans; Chemistry; Chemistry, Applied; Chemistry, Medicinal; China; Cholestanols - chemistry; Cholestanols - isolation & purification; Chromatography, High Pressure Liquid; high performance liquid chromatography; Life Sciences & Biomedicine; metabolites; Molecular Structure; nuclear magnetic resonance spectroscopy; Nuclear Magnetic Resonance, Biomolecular; Pharmacology & Pharmacy; Physical Sciences; Plant Sciences; polyhydroxycholestane sulfate; Science & Technology; Skin; Skin - chemistry; sulfates; toad skin; toad venom; toads; Venoms; China; toad skin; Bufo bufo gargarizans; STEROIDS; polyhydroxycholestane sulfate; toad venom; IDENTIFICATION; bufadienolide; CANCER; MASS-SPECTROMETRY; CYTOTOXIC BUFADIENOLIDES; TOAD; SKIN; CONSTITUENTS
• ISSN: 1477-2213; 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2014.995174

A new polyhydroxycholestane sulfate ester, 3α,12β,25,26-tetrahydroxy-7- oxo -5β - cholestane 26- O -sulfate (1), was isolated from dried skin of Bufo bufo gargarizans Cantor and its structure was elucidated on the basis of extensive 1D and 2D NMR as well as HR-ESI-MS analysis. A comparison of steroidal metabolite profiles, based on HPLC and LC–MS analyses, indicates that the chemical compositions of the various parts of toads, such as venom, skin and stratum corneum, are significantly different.