Novel highly hydrophilic methacrylate-based monolithic column with mixed-mode of hydrophilic and strong cation-exchange interactions for pressurized capillary electrochromatography

• Published in: Journal of Chromatography A Vol. 1218; no. 29; pp. 4671 - 4677
• Main Authors: Lin, Jia, Liu, Shaofeng, Lin, Jian, Lin, Xucong, Xie, Zenghong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22.07.2011; Elsevier
• Subjects: acetonitrile; Acetonitriles - chemistry; Acrylamides - chemistry; Acrylates - chemistry; Alkanesulfonates - chemistry; amides; Analytical chemistry; Capillary Electrochromatography - instrumentation; cation exchange; Cation Exchange Resins - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Ion Exchange - instrumentation; Exact sciences and technology; Hydrogen-Ion Concentration; Hydrophilic interaction; hydrophilic interaction chromatography; hydrophilicity; Hydrophobic and Hydrophilic Interactions; Methacrylate-based monolith; Methacrylates - chemistry; Microscopy, Electron, Scanning; Osmolar Concentration; Other chromatographic methods; peptides; Peptides - chemistry; permeability; phenols; Pressurized capillary electrochromatography; Propylene Glycols - chemistry; solutes; solvents; Strong cation-exchange; Methacrylate-based monolith; Hydrophilic interaction; Strong cation-exchange; Pressurized capillary electrochromatography; Performance evaluation; Peak resolution; Peptides; Pressurized capillary; Acrylamide derivative copolymer; Mobile phase; Mechanism; Surface structure; Sulfonate copolymer; Characterization; Crosslinked copolymer; Plate efficiency; Stationary phase; Scanning electron microscopy; Phase composition; Hydrophilic Interaction Liquid chromatography; Monolithic column; Operating conditions; Pressure effect; Electrophoretic mobility; Morphology; Preparation; Electrochromatography; Phenols; Carboxamide; Height equivalent to a theoretical plate; Methacrylate copolymer; Radical copolymerization; Mixed adsorbent; Cation exchanger
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2011.05.052

A novel highly hydrophilic polymethacrylate-based monolithic stationary phase based on the copolymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) and pentaerythritol triacrylate (PETA) was designed for pressurized capillary electrochromatography. A typical hydrophilic interaction chromatography mechanism could be observed when the content of acetonitrile (ACN) in the mobile phase exceeded 25%. Slight swelling or shrinking with mobile phases of different polarity was observed in permeability studies. Good retentions and efficient separations of polar analytes, such as neutral amides and phenols, were well achieved in hydrophilic interaction chromatography mode with only about 50% ACN content in the mobile phase. It was remarkably lower than the content of ACN (>90%) used on the hydrophilic polymethacrylate-based monoliths reported previously. Additionally, a mixed mode of hydrophilic interaction (HI) and strong cation-exchange (SCX) could be also obtained in the analysis of charged peptides, and high column efficiency up to 80,000 plates/m was achieved without peak tailing. The prepared hydrophilic stationary phase might provide a potential environmental friendly separation media for polar solutes as it consumes a low volume of organic solvents.