Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight

• Published in: Molecular breeding Vol. 30; no. 4; pp. 1601 - 1610
• Main Authors: Luo, Yanchang, Sangha, Jatinder S., Wang, Shouhai, Li, Zefu, Yang, Jianbo, Yin, Zhongchao
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2012; Springer Nature B.V
• Subjects: Agronomy; Bacteria; Bacterial diseases; Biomedical and Life Sciences; Biotechnology; Blight; Breeding; Crop production; Crop yield; Disease resistance; Fertility; Genes; Genetic crosses; Heterosis; High resistance; Hybrids; Inbreeding; Life Sciences; Marker-assisted selection; Molecular biology; Offspring; Oryza; Pedigree; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Progeny; Rice; Hybrid rice; Disease resistance; Gene pyramiding; Marker-assisted selection; pv; Bacterial blight
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-012-9742-7

Hybrid rice based on heterosis can significantly increase rice yield compared to inbred rice. Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv . oryzae ( Xoo ) is one of the most destructive bacterial diseases that affect hybrid rice production. To breed a broad-spectrum and high disease resistance to BB in hybrid rice, we introduced the Xa4 , Xa21 and Xa27 genes into the restorer lines of Mianhui 725 or 9311 genetic backgrounds and pyramided the three R genes in the progeny derived from the cross between the two lines. A near-isogenic line of the Xa27 gene in the genetic background of 9311 [9311( Xa27 )] and another line with the Xa4 and Xa21 genes in the genetic background of Mianhui 725 (WH421) were firstly developed through marker-assisted selection. A new restorer line carrying Xa4, Xa21 and Xa27 , designated as XH2431, was selected from the F8 progeny of the cross between 9311( Xa27 ) and WH421 through marker-assisted breeding and pedigree selection. XH2431 and II You 2431, the hybrids derived from cytoplasmic male-sterile line II-32A and restorer line XH2431, conferred high resistance to all 23 Xoo strains collected from 10 countries. XH2431 restored the fertility of II-32A to the normal level in the F1 generation. In addition, II You 2431 showed good agronomic traits under greenhouse conditions. The development of XH2431, 9311( Xa27 ) and WH421 provides a set of restorer lines with broad-spectrum and enhanced resistance to BB for hybrid rice.