Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight
Hybrid rice based on heterosis can significantly increase rice yield compared to inbred rice. Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv . oryzae ( Xoo ) is one of the most destructive bacterial diseases that affect hybrid rice production. To breed a broad-spectrum and high diseas...
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Published in | Molecular breeding Vol. 30; no. 4; pp. 1601 - 1610 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.12.2012
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Hybrid rice based on heterosis can significantly increase rice yield compared to inbred rice. Bacterial blight (BB) of rice caused by
Xanthomonas oryzae
pv
. oryzae
(
Xoo
) is one of the most destructive bacterial diseases that affect hybrid rice production. To breed a broad-spectrum and high disease resistance to BB in hybrid rice, we introduced the
Xa4
,
Xa21
and
Xa27
genes into the restorer lines of Mianhui 725 or 9311 genetic backgrounds and pyramided the three
R
genes in the progeny derived from the cross between the two lines. A near-isogenic line of the
Xa27
gene in the genetic background of 9311 [9311(
Xa27
)] and another line with the
Xa4
and
Xa21
genes in the genetic background of Mianhui 725 (WH421) were firstly developed through marker-assisted selection. A new restorer line carrying
Xa4, Xa21
and
Xa27
, designated as XH2431, was selected from the F8 progeny of the cross between 9311(
Xa27
) and WH421 through marker-assisted breeding and pedigree selection. XH2431 and II You 2431, the hybrids derived from cytoplasmic male-sterile line II-32A and restorer line XH2431, conferred high resistance to all 23
Xoo
strains collected from 10 countries. XH2431 restored the fertility of II-32A to the normal level in the F1 generation. In addition, II You 2431 showed good agronomic traits under greenhouse conditions. The development of XH2431, 9311(
Xa27
) and WH421 provides a set of restorer lines with broad-spectrum and enhanced resistance to BB for hybrid rice. |
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ISSN: | 1380-3743 1572-9788 |
DOI: | 10.1007/s11032-012-9742-7 |