Performance enhancement of polyaniline-based polymeric wire biosensor

• Published in: Biosensors & bioelectronics Vol. 24; no. 5; pp. 1348 - 1352
• Main Authors: Yuk, Jong Seol, Jin, Joon-Hyung, Alocilja, Evangelyn C., Rose, Joan B.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier B.V 2009; Elsevier
• Subjects: Aniline Compounds - chemistry; Biological and medical sciences; Biosensors; Biotechnology; Electrochemistry - instrumentation; Electrodes; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Immunoassay - instrumentation; Immunoglobulin G - analysis; Methods. Procedures. Technologies; Polyaniline; Polymeric wire biosensor; Pulse mode; Reproducibility of Results; Screen-printing; Sensitivity and Specificity; Streptavidin - chemistry; Various methods and equipments; Polymeric wire biosensor; Polyaniline; Screen-printing; Pulse mode; Biosensor; Polymer; Aniline polymer
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2008.07.079

We demonstrate here the performance enhancement of polyaniline-based biosensor using screen-printing technology and pulse mode measurement technique. Screen-printed silver electrodes were made on a nitrocellulose membrane and the distance between the two electrodes was approximately 550 μm. Resistance of the electrodes had an average of 1.4 Ω with a standard deviation of ±0.4 Ω. The surface of nitrocellulose membrane was modified by glutaraldehyde to immobilize streptavidin. Biotinylated anti-mouse IgG was conjugated with polyaniline-coated magnetic nanoparticles. Formation of polyaniline-coated magnetic nanoparticles was confirmed by a transmission electron microscope image. The polyaniline was used as an electric signal transducer for the monitoring of the biospecific binding event. An electrical response induced by the streptavidin–biotin interaction was measured by pulse mode measurement. This measurement method reduced the resistance caused by interfacial capacitance. Dose-dependent resistance changes were also successfully analyzed by the pulse mode polymeric wire biosensor. Results showed that the pulse mode measurement technique enhanced the performance of the polyaniline-based polymeric wire biosensor by reducing the interfacial effects. This approach could be helpful in samples with high interfering background materials, such as food and clinical specimens.