Enhanced tolerance to NaCl and LiCl stresses by over-expressing Caragana korshinskii sodium/proton exchanger 1 (CkNHX1) and the hydrophilic C terminus is required for the activity of CkNHX1 in Atsos3-1 mutant and yeast

• Published in: Biochemical and biophysical research communications Vol. 417; no. 2; pp. 732 - 737
• Main Authors: Yang, Da-Hai, Song, Li-Ying, Hu, Jun, Yin, Wei-Bo, Li, Zhi-Guo, Chen, Yu-Hong, Su, Xiao-Hua, Wang, Richard R.-C., Hu, Zan-Min
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 13.01.2012
• Subjects: 60 APPLIED LIFE SCIENCES; Antiporter; ARABIDOPSIS; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Caragana; Caragana - genetics; Caragana - physiology; Caragana korshinskii; DROUGHTS; Exchanger; Hydrophobic and Hydrophilic Interactions; Lithium Chloride - metabolism; LITHIUM CHLORIDES; LITHIUM IONS; MUTANTS; NHX; PEAS; Plants, Genetically Modified; Protein Structure, Tertiary; ROOTS; Saccharomyces cerevisiae - genetics; Salt tolerance; Salt-Tolerance - genetics; SHRUBS; Sodium Chloride - metabolism; SODIUM CHLORIDES; SODIUM IONS; Sodium-Hydrogen Exchangers - biosynthesis; Sodium-Hydrogen Exchangers - chemistry; Sodium-Hydrogen Exchangers - genetics; SOS; Stress, Physiological - genetics; TOLERANCE; YEASTS; Salt tolerance; Caragana korshinskii; Antiporter; Exchanger; SOS; NHX
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2011.12.023

► CkNHX1 was isolated from Caragana korshinskii. ► CkNHX1 was expressed mainly in roots, and significantly induced by NaCl in stems. ► Expression of CkNHX1 enhanced the resistance to NaCl and LiCl in yeast and Atsos3-1. ► Expression of CkNHX1-ΔC had little effect on NaCl/LiCl tolerance in Atsos3-1. ► C-terminal region of CkNHX1 is required for its Na+ and Li+ transporting activity. Sodium/proton exchangers (NHX antiporters) play important roles in plant responses to salt stress. Previous research showed that hydrophilic C-terminal region of Arabidopsis AtNHX1 negatively regulates the Na+/H+ transporting activity. In this study, CkNHX1 were isolated from Caragana korshinskii, a pea shrub with high tolerance to salt, drought, and cold stresses. Transcripts of CkNHX1 were detected predominantly in roots, and were significantly induced by NaCl stress in stems. Transgenic yeast and Arabidopsisthalianasos3-1 (Atsos3-1) mutant over-expressing CkNHX1 and its hydrophilic C terminus-truncated derivative, CkNHX1-ΔC, were generated and subjected to NaCl and LiCl stresses. Expression of CkNHX1 significantly enhanced the resistance to NaCl and LiCl stresses in yeast and Atsos3-1 mutant. Whereas, compared with expression of CkNHX1, the expression of CkNHX1-ΔC had much less effect on NaCl tolerance in Atsos3-1 and LiCl tolerance in yeast and Atsos3-1. All together, these results suggest that the predominant expression of CkNHX1 in roots might contribute to keep C. korshinskii adapting to the high salt condition in this plant’s living environment; CkNHX1 could recover the phenotype of Atsos3-1 mutant; and the hydrophilic C-terminal region of CkNHX1 should be required for Na+/H+ and Li+/H+ exchanging activity of CkNHX1.