Characterization of a Read-through Fusion Transcript, BCL2L2-PABPN1, Involved in Porcine Adipogenesis

• Published in: Genes Vol. 13; no. 3; p. 445
• Main Authors: Zhu, Jiyuan, Yang, Zewei, Hao, Wanjun, Li, Jiaxin, Wang, Liang, Xia, Jiqiao, Zhang, Dongjie, Liu, Di, Yang, Xiuqin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.02.2022; MDPI
• Subjects: 1-Phosphatidylinositol 3-kinase; Adenoviruses; Adipogenesis; Adipogenesis - genetics; AKT protein; Animals; Cell cycle; Gene expression; Genomes; Hogs; MAP kinase; Metabolic disorders; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Morphology; Phosphatidylinositol 3-Kinases - metabolism; Polymerase chain reaction; Pork; Preadipocytes; Proteins; RNA Splicing; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal transduction; Swine - genetics; Transcription; Tumors; Wnt protein; Beijing China; United States > US; China; Germany; adipogenesis; genome-wide analysis; BCL2L2-PABPN1; cis-SAGe; RNA-Seq; chimeric RNA
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes13030445

-Splicing of adjacent genes ( -SAGe) has been involved in multiple physiological and pathological processes in humans. However, to the best of our knowledge, there is no report of -SAGe in adipogenic regulation. In this study, a -SAGe product, BCL2L2-PABPN1 (BP), was characterized in fat tissue of pigs with RT-PCR and RACE method. BP is an in-frame fusion product composed of 333 aa and all the functional domains of both parents. BP is highly conserved among species and rich in splicing variants. BP was found to promote proliferation and inhibit differentiation of primary porcine preadipocytes. A total of 3074/44 differentially expressed mRNAs (DEmRs)/known miRNAs (DEmiRs) were identified in porcine preadipocytes overexpressing BP through RNA-Seq analysis. Both DEmRs and target genes of DEmiRs were involved in various fat-related pathways with MAPK and PI3K-Akt being the top enriched. , , and were hub genes among the fat-related pathways identified. Moreover, ssc-miR-339-3p was found to be critical for BP regulating adipogenesis through integrated analysis of mRNA and miRNA data. The results highlight the role of -SAGe in adipogenesis and contribute to further revealing the mechanisms underlying fat deposition, which will be conductive to human obesity control.