Evaluation of different strategies for the development of amperometric biosensors for l-lactate
Two strategies were investigated for the development of lactate biosensors based on sol–gel matrixes and polysulfone composite films, both containing l-lactate dehydrogenase (LDH). Firstly, reagentless disposable screen-printed electrodes (SPE's) with Meldola's Blue (MB) and the cofactor N...
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Published in | Biosensors & bioelectronics Vol. 22; no. 11; pp. 2663 - 2668 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
15.05.2007
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Two strategies were investigated for the development of lactate biosensors based on sol–gel matrixes and polysulfone composite films, both containing
l-lactate dehydrogenase (LDH). Firstly, reagentless disposable screen-printed electrodes (SPE's) with Meldola's Blue (MB) and the cofactor NAD
+ inside a sol–gel matrix were prepared. These showed relatively low sensitivities (260
μA/M). Secondly, mediator-modified-polysulfone–graphite composite films deposited over both cylindrical epoxy–graphite and SPE's. These electrodes showed enhanced performance characteristics: improved sensitivity (80
mA/M), detection limit (0.87
μM) and reproducibility (2%). Reagentless electrodes, incorporating NAD
+ in the polysulfone film, had a decreased sensitivity, although better than that achieved by the sol–gel electrodes. While sol–gel electrodes showed a linear range between 1.25
×
10
−4 and 2.48
×
10
−3
M, the epoxy–graphite composite electrodes based on polysulfone composite films allowed the detection of lactate at a linear range of lower concentrations from 1
×
10
−6 to 1.2
×
10
−5
M. Finally, the performance of the LDH-MB-polysulfone-composite film-based SPE's in a flow system was studied. Short response times were obtained (
t
<
30
s). Furthermore, repeatability and reproducibility values were notably improved, especially when working with electrodes covered with a polyamide layer prepared with
N-(2-aminoethyl)-piperazine. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0956-5663 1873-4235 |
DOI: | 10.1016/j.bios.2006.10.034 |