Cry1Ab protein from Bt transgenic rice does not residue in rhizosphere soil

• Published in: Environmental pollution (1987) Vol. 143; no. 3; pp. 449 - 455
• Main Authors: Wang, Haiyan, Ye, Qingfu, Wang, Wei, Wu, Licheng, Wu, Weixiang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2006
• Subjects: Bacillus thuringiensis; Bacteria - genetics; Bacterial Proteins - analysis; Bacterial Proteins - genetics; Bacterial Toxins - analysis; Bacterial Toxins - genetics; biodegradation; Bt transgenic rice; Cry1Ab protein; Degradation; Endotoxins - analysis; Endotoxins - genetics; Environmental Monitoring - methods; Environmental Pollution; gene expression; half life; Hemolysin Proteins - analysis; Hemolysin Proteins - genetics; Humans; Oryza - genetics; Oryza - metabolism; Oryza - microbiology; Oryza sativa; Pest Control, Biological; plant residues; Plant Roots - metabolism; Plants, Genetically Modified - metabolism; protein synthesis; Residue; rhizosphere; rice; rice straw; Soil; Soil - analysis; soil amendments; Soil Microbiology; soil types; transgenic plants; China; Degradation; Soil; Bt transgenic rice; Residue; Cry1Ab protein
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2005.12.006

Expression of Cry1Ab protein in Bt transgenic rice (KMD) and its residue in the rhizosphere soil during the whole growth in field, as well as degradation of the protein from KMD straw in five soils under laboratory incubation were studied. The residue of Cry1Ab protein in KMD rhizosphere soil was undetectable (below the limit of 0.5 ng/g air-dried soil). The Cry1Ab protein contents in the shoot and root of KMD were 3.23–8.22 and 0.68–0.89 μg/g (fresh weight), respectively. The half-lives of the Cry1Ab protein in the soils amended with KMD straw (4%, w/w) ranged from 11.5 to 34.3 d. The residence time of the protein varied significantly in a Fluvio-marine yellow loamy soil amended with KMD straw at the rate of 3, 4 and 7%, with half-lives of 9.9, 13.8 and 18 d, respectively. In addition, an extraction method for Cry1Ab protein in soil was developed, with extraction efficiencies of 46.4–82.3%. Cry1Ab protein was not detected in the rhizosphere soil of field-grown Bt transgenic rice.