Strategies to develop malic acid biosensors based on malate quinone oxidoreductase (MQO)

• Published in: Biosensors & bioelectronics Vol. 21; no. 12; pp. 2290 - 2297
• Main Authors: Bucur, Bogdan, Mallat, Elena, Gurban, Ana-Maria, Gocheva, Yana, Velasco, Carine, Marty, Jean-Louis, Noguer, Thierry
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.06.2006; Elsevier Science
• Subjects: Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensor; Biosensors; Biotechnology; Electrochemistry - instrumentation; Electrochemistry - methods; Electrodes; Equipment Design; Equipment Failure Analysis; Food Analysis - methods; Fundamental and applied biological sciences. Psychology; Malate quinone oxidoreductase; Malates - analysis; Malates - chemistry; Malic acid; Methods. Procedures. Technologies; Microchemistry - instrumentation; Microchemistry - methods; Oxidoreductases - chemistry; Screen-printed electrode (SPE); Various methods and equipments; Vitaceae; Wine - analysis; Malic acid; Wine analysis; Malate quinone oxidoreductase; Biosensor; Screen-printed electrode (SPE); Wine; NADPH:quinone reductase; Enzyme; Amperometry; Oxidoreductases; Enzyme electrode
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2005.10.022

An amperometric biosensor based on malate quinone oxidoreductase (MQO) was developed for monitoring of the malolactic fermentation of wines. Screen-printed electrodes coupled with appropriate mediators were used as transducers for this novel biosensor. MQO was immobilized by physical entrapment in a photo-cross-linkable poly(vinyl alcohol) polymer (PVA-SbQ) on the surface of the working electrode. Several electrochemical mediators were studied in order to lower the applied potential and minimise the matrix effects. Among them, 2,6-dichlorophenol indophenol (DPIP) and phenazine methosulfate (PMS) were chosen for further development. The working conditions (mediator concentration, applied potential and pH) were optimised for both DPIP and PMS. Detection limits for both types of biosensors were of 5 μM malic acid. Sensitivities obtained for the linear part of the calibration curve were 0.85 and 1.7 mA/M for the biosensors based on DPIP and PMS, respectively. Interferences due to non-specific oxidations were shown to be negligible when using PMS as mediator.